##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512639_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2143619 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 30.88529491481462 31.0 31.0 34.0 27.0 34.0 2 31.05806209032482 31.0 31.0 34.0 27.0 34.0 3 31.142434826338075 33.0 31.0 34.0 27.0 34.0 4 34.678929884461745 37.0 35.0 37.0 32.0 37.0 5 34.39883113557027 37.0 35.0 37.0 31.0 37.0 6 34.28645762143366 37.0 35.0 37.0 30.0 37.0 7 34.23610072498891 37.0 35.0 37.0 30.0 37.0 8 34.20457553324541 37.0 35.0 37.0 30.0 37.0 9 35.698615285645445 39.0 37.0 39.0 30.0 39.0 10 35.45235557251545 39.0 35.0 39.0 30.0 39.0 11 35.5814125551229 39.0 35.0 39.0 30.0 39.0 12 35.48889984647458 39.0 35.0 39.0 30.0 39.0 13 35.487058567777204 39.0 35.0 39.0 30.0 39.0 14 36.58982916273834 40.0 37.0 41.0 30.0 41.0 15 36.54876869443684 40.0 37.0 41.0 30.0 41.0 16 36.50321862233914 40.0 37.0 41.0 30.0 41.0 17 36.46547590779891 40.0 37.0 41.0 30.0 41.0 18 36.385632428150714 40.0 36.0 41.0 29.0 41.0 19 36.42288671634278 40.0 37.0 41.0 29.0 41.0 20 36.3648633455852 40.0 37.0 41.0 29.0 41.0 21 36.3102598922663 40.0 37.0 41.0 29.0 41.0 22 36.19002863848473 40.0 36.0 41.0 29.0 41.0 23 36.113681115907255 40.0 36.0 41.0 29.0 41.0 24 36.053170362830336 40.0 36.0 41.0 29.0 41.0 25 35.95751670422776 40.0 36.0 41.0 29.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.7900436426273352 1101 2 0.7198060326859022 1101 3 0.7319104432960906 1101 4 0.7584055797247515 1101 5 0.7209410887336745 1101 6 0.9029076696779086 1101 7 0.7692233217459403 1101 8 0.7740575932717704 1101 9 0.9262013034751533 1101 10 1.0309439631355062 1101 11 0.9838253733238673 1101 12 1.004007293374606 1101 13 0.9001599175401722 1101 14 1.0166356656168105 1101 15 1.2024598744197803 1101 16 1.186963329763131 1101 17 1.2240717236598968 1101 18 1.1500283201625408 1101 19 1.2744624454284263 1101 20 1.2261906624074044 1101 21 1.1546213301768873 1101 22 1.3745316344738043 1101 23 1.3576560112573262 1101 24 1.3373901557432575 1101 25 1.4042380525544615 1102 1 0.5579341958781185 1102 2 0.6080445185036361 1102 3 0.6562729012032165 1102 4 0.5220690056360127 1102 5 0.660793724090631 1102 6 0.7016217125994473 1102 7 0.6050992229820764 1102 8 0.6652946751140405 1102 9 0.63412774782811 1102 10 0.8701832828935565 1102 11 0.8156894938335242 1102 12 0.6561629763083872 1102 13 0.886668241109021 1102 14 1.0326471085446798 1102 15 1.0210934790476216 1102 16 0.9305273162089662 1102 17 1.0166861793291062 1102 18 1.048990856185597 1102 19 1.107606611540895 1102 20 1.0307648778173615 1102 21 0.9726395079645158 1102 22 1.084896773657384 1102 23 0.9445295795718209 1102 24 0.945696201282729 1102 25 0.9482078195789825 1103 1 0.4999014640839192 1103 2 0.45121284864444533 1103 3 0.4702272648823502 1103 4 0.4711162886282523 1103 5 0.5259696490830734 1103 6 0.6323082737447194 1103 7 0.5655817985105216 1103 8 0.6185103890637009 1103 9 0.6832671099949934 1103 10 0.8495306633418522 1103 11 0.7001859148437077 1103 12 0.6745478941428402 1103 13 0.6563614225453165 1103 14 0.8869281180550459 1103 15 0.8489806988614461 1103 16 0.7601028782615913 1103 17 0.9472396625431401 1103 18 0.9955701484529627 1103 19 0.9248005491018034 1103 20 0.9286833507817036 1103 21 0.8752183969654226 1103 22 0.7953111299433004 1103 23 0.7536622206310639 1103 24 0.9185872857334445 1103 25 0.8229062098737003 1104 1 0.3218309415307523 1104 2 0.40770002359811386 1104 3 0.388254923463613 1104 4 0.37450564822255217 1104 5 0.23299505594935255 1104 6 0.4712911010268641 1104 7 0.3933732187935419 1104 8 0.4279941597835446 1104 9 0.3927328258336189 1104 10 0.5628590011795538 1104 11 0.5307319674198965 1104 12 0.5308948397340387 1104 13 0.5374379424997073 1104 14 0.5645820686595115 1104 15 0.6474633404496046 1104 16 0.6742381663866226 1104 17 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0.9303402381166492 1107 17 0.9645200744447919 1107 18 0.883936824597896 1107 19 0.9480043351805136 1107 20 1.001936216868998 1107 21 0.958193571523573 1107 22 1.0688992217496107 1107 23 1.0435589292744893 1107 24 1.0101822389358475 1107 25 1.0295575587840844 1108 1 0.29074473312924454 1108 2 0.32655466403893385 1108 3 0.40290862454319054 1108 4 0.30175696728407786 1108 5 0.5304510918746246 1108 6 0.5691594307356027 1108 7 0.49470035905329013 1108 8 0.4038101578966504 1108 9 0.411567965892786 1108 10 0.4592215299890441 1108 11 0.5719149162861825 1108 12 0.5241339645569099 1108 13 0.49999553826979337 1108 14 0.7060434086885934 1108 15 0.6759704840894116 1108 16 0.8296058785832798 1108 17 0.8008871648136946 1108 18 0.73324063935992 1108 19 0.7616849356452349 1108 20 0.6813471143145975 1108 21 0.7076505160371056 1108 22 0.6582858303942274 1108 23 0.4739324750284908 1108 24 0.571745589881985 1108 25 0.6613079024969295 1109 1 -0.10261400854241032 1109 2 -0.04824321728010972 1109 3 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-0.7521467906531889 2315 3 -0.807255871980729 2315 4 -0.6547926229497563 2315 5 -0.8364773552494142 2315 6 -0.7060822702769514 2315 7 -0.9136484599035413 2315 8 -0.9602375186940151 2315 9 -1.120362999076434 2315 10 -1.0135097621787992 2315 11 -0.9555789022624595 2315 12 -1.1924496226490433 2315 13 -1.2308185218949887 2315 14 -1.3927939368963678 2315 15 -1.4077896974217623 2315 16 -1.459297658443596 2315 17 -1.4817415625469152 2315 18 -1.4272067207016406 2315 19 -1.165088205291859 2315 20 -1.3087459934176522 2315 21 -1.2780393937488626 2315 22 -1.1477241003427991 2315 23 -1.4014794533337636 2315 24 -1.2948926786164918 2315 25 -1.2827805769266547 2316 1 -1.5431527348123382 2316 2 -1.4257465362565789 2316 3 -1.5159502941036784 2316 4 -1.4573357305969168 2316 5 -1.6814435980585856 2316 6 -4.709448098646579 2316 7 -2.742320405847245 2316 8 -2.10104805704367 2316 9 -2.376898054563334 2316 10 -1.9430438424040517 2316 11 -2.094719216398971 2316 12 -2.321912340445344 2316 13 -2.3395532906631757 2316 14 -2.59668725752978 2316 15 -2.6658018282882168 2316 16 -2.5198571891535124 2316 17 -2.7030862068749997 2316 18 -2.772571018063502 2316 19 -2.9372003876273425 2316 20 -2.812920247629883 2316 21 -3.050683248574096 2316 22 -3.07613250543357 2316 23 -3.1684281235180407 2316 24 -3.1266652698452617 2316 25 -3.3125340816915525 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37459.0 3 1.0 4 711.0 5 5773.0 6 6798.0 7 5339.0 8 4160.0 9 3779.0 10 3494.0 11 3190.0 12 3056.0 13 2893.0 14 2878.0 15 2883.0 16 2934.0 17 3124.0 18 3313.0 19 3866.0 20 4900.0 21 6239.0 22 8067.0 23 9765.0 24 11637.0 25 13545.0 26 15575.0 27 17818.0 28 20391.0 29 24356.0 30 31025.0 31 39967.0 32 52103.0 33 67826.0 34 90022.0 35 123983.0 36 191822.0 37 387788.0 38 931139.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 40.920717785060596 20.82008288459311 14.63835150092862 23.620847829417677 2 15.649111216554854 21.726403346579122 40.43207673318487 22.192408703681153 3 19.55855961343877 25.532848887792092 30.757984511240107 24.150606987529034 4 13.607484361318049 18.397499863544947 37.953351499349445 30.041664275787554 5 12.835732817160686 38.64590584424528 35.88469031742096 12.63367102117307 6 31.302783462095775 38.264682068381376 17.63150219218932 12.801032277333531 7 27.448137746257924 32.14085573010304 22.462203628433006 17.94880289520603 8 24.59032839520344 38.328109638335526 20.33550166836279 16.74606029809824 9 26.20030039880531 14.977946024335195 20.573212060186147 38.24854151667335 10 15.13606608704254 29.415061175029035 34.70325643118212 20.74561630674631 11 33.84633403826017 22.17234434524865 25.595330310796204 18.385991305694976 12 23.49835350025268 24.93244381439186 32.21035999945871 19.35884268589675 13 29.166487810067355 22.3386138022549 26.017382995123278 22.477515392554466 14 20.29062115536512 23.27162011543294 27.891457020102088 28.546301709099847 15 22.30779673541707 32.61279997536797 23.44404215248436 21.635361136730594 16 21.229270504636204 28.660585828088177 28.959573868566558 21.150569798709064 17 20.977069807561417 29.561301982232635 28.42932403679243 21.032304173413515 18 20.895044738241634 27.539396710176433 31.32567947079185 20.23987908079008 19 23.376099316851434 26.86797561489902 28.447465110160273 21.308459958089273 20 23.428183833041228 27.639426595864286 28.94567551416553 19.98671405692896 21 22.28479034753844 26.855471984527103 28.079430159930475 22.78030750800399 22 22.916751220254046 28.694198714225667 28.0701582516604 20.318891813859885 23 21.46626802617443 28.3951112581107 28.91927156831508 21.219349147399793 24 22.84128981936147 27.024964335996433 29.45776719546113 20.675978649180966 25 22.139475345198935 27.8654928884284 29.067059024947994 20.927972741424668 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 189.0 1 189.0 2 466.5 3 744.0 4 744.0 5 744.0 6 1850.5 7 2957.0 8 2957.0 9 2957.0 10 4345.0 11 5733.0 12 5733.0 13 5733.0 14 8544.5 15 11356.0 16 11356.0 17 11356.0 18 20238.0 19 29120.0 20 29120.0 21 29120.0 22 45747.0 23 62374.0 24 62374.0 25 62374.0 26 90169.5 27 117965.0 28 117965.0 29 117965.0 30 151695.0 31 185425.0 32 185425.0 33 185425.0 34 205106.5 35 224788.0 36 224788.0 37 224788.0 38 235926.0 39 247064.0 40 247064.0 41 247064.0 42 256728.0 43 266392.0 44 266392.0 45 266392.0 46 270755.0 47 275118.0 48 275118.0 49 275118.0 50 260227.0 51 245336.0 52 245336.0 53 245336.0 54 215254.0 55 185172.0 56 185172.0 57 185172.0 58 157099.0 59 129026.0 60 129026.0 61 129026.0 62 104747.5 63 80469.0 64 80469.0 65 80469.0 66 61661.0 67 42853.0 68 42853.0 69 42853.0 70 31386.0 71 19919.0 72 19919.0 73 19919.0 74 13848.5 75 7778.0 76 7778.0 77 7778.0 78 5261.0 79 2744.0 80 2744.0 81 2744.0 82 1784.5 83 825.0 84 825.0 85 825.0 86 525.5 87 226.0 88 226.0 89 226.0 90 130.0 91 34.0 92 34.0 93 34.0 94 21.0 95 8.0 96 8.0 97 8.0 98 6.0 99 4.0 100 4.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.1311333777131104 2 0.013808423978328238 3 0.0 4 0.0026124045364404776 5 0.07930513771337164 6 0.036573663510166686 7 0.022858539693854176 8 0.023325040503932835 9 0.021832237911681134 10 0.02360494098998003 11 0.028316599171774464 12 0.027430247632625013 13 0.017493780377949627 14 0.00979651701165179 15 0.0031722055085348657 16 0.002565754455432612 17 0.0012129021062045075 18 0.001819353159306761 19 6.99751215117985E-4 20 0.0 21 0.0 22 3.732006480629254E-4 23 0.0 24 4.6650081007865674E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2143619.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 68.6884525366045 #Duplication Level Percentage of deduplicated Percentage of total 1 83.16087203856323 57.121916119234896 2 11.383468891370176 15.638257252935883 3 2.8437034899284095 5.859887765883723 4 1.0100325454455363 2.7751029023304614 5 0.4826844698087512 1.6577424647307257 6 0.28640432399609633 1.1803601889050492 7 0.17126041404724363 0.823452897517833 8 0.12920198782617226 0.7099747686746388 9 0.0827837038994911 0.5117656064494063 >10 0.40110642439759464 4.745220193044842 >50 0.024219316693042323 1.1546863254715007 >100 0.019711866932580344 2.838076055123322 >500 0.0023771410180743356 1.1750715690750928 >1k 0.0018337944996570461 2.365911527476073 >5k 3.3959157401056405E-4 1.4425743631465695 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8663 0.4041296517711403 No Hit TATCAACGCAGAGTACTTTTTTTTT 5904 0.2754220782704389 No Hit GGTATCAACGCAGAGTACTTTTTTT 5873 0.2739759257591951 No Hit CTGTAGGACGTGGAATATGGCAAGA 5353 0.24971788363510491 No Hit GTCCTACAGTGGACATTTCTAAATT 5129 0.23926826548934305 No Hit CTTTAGGACGTGAAATATGGCGAGG 4760 0.22205438559744062 No Hit GTCCTAAAGTGTGTATTTCTCATTT 4623 0.21566332449936298 No Hit GTCCTACAGTGTGCATTTCTCATTT 2881 0.134398883383661 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2811 0.1311333777131104 No Hit CTGAAGGACCTGGAATATGGCGAGA 2765 0.12898747398674856 No Hit CTGTAGGACCTGGAATATGGCGAGA 2467 0.1150857498464046 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 4.665008100786567E-5 7 0.0 0.0 0.0 0.0 4.665008100786567E-5 8 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 9 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 10 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 11 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 12 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 13 0.0 0.0 0.0 4.665008100786567E-5 4.665008100786567E-5 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CCGCGCC 60 4.0973088E-4 12.665142 9 TCCAACG 105 2.00871E-8 12.660416 18 GGTATCA 1790 0.0 12.651314 1 TAGGACC 990 0.0 12.097922 4 GGCGAGG 1290 0.0 11.703524 19 CCAACGA 90 7.5052685E-6 11.60538 19 CTAGGAC 305 0.0 11.219358 3 ACTGCGC 60 0.0058821575 11.081999 8 CACCTTT 755 0.0 10.943149 14 GATATAC 510 0.0 10.821054 1 TGTAGGA 3190 0.0 10.788077 2 AGGACGT 3030 0.0 10.603507 5 CCACCTT 800 0.0 10.565013 13 ATAGGAC 370 0.0 10.532887 3 GTAGGAC 3070 0.0 10.496068 3 TAGGACA 980 0.0 10.475461 4 ATTTAGA 745 0.0 10.472956 1 GGACGTG 3030 0.0 10.440312 6 CTGTAGG 3205 0.0 10.33149 1 TTAGACT 175 2.3283064E-10 10.320269 4 >>END_MODULE