FastQCFastQC Report
Thu 26 May 2016
SRR1512638_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512638_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755199
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT45180.5982529108221807No Hit
GGTATCAACGCAGAGTACTTTTTTT31570.41803551116990356No Hit
TATCAACGCAGAGTACTTTTTTTTT28180.3731466805438037No Hit
ACGCAGAGTACTTTTTTTTTTTTTT13560.1795553225043995No Hit
GTACTTTTTTTTTTTTTTTTTTTTT12870.17041865786368893No Hit
GAGTACTTTTTTTTTTTTTTTTTTT9900.1310912752797607No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGTGT350.002234164216.2134748
GGTATCA6650.016.0796411
GCACCGT400.00532220414.2311776
AGCGTAC500.001175895713.7340771
GTCCTAT550.002409717312.4855271
GTTATAT802.059144E-512.2625711
CTCGAAC658.1633404E-411.66676818
GTATTAC600.00462106911.4450661
TAGACCA1701.2732926E-1111.1617084
TATTACA855.3861644E-511.1617082
CTATACT600.005928908511.0686944
TACACGG600.005928908511.0686945
CAGTGCG600.006056397711.0371019
CCTCGAA803.800348E-410.67693717
GTATAGA650.008381451510.5646761
TCCTACA1803.6379788E-1110.5416142
CTAGTCT909.627275E-510.5416144
ATACTGC1201.5452624E-610.2780726
GTGTAGA1052.8766923E-510.2772021
GTAGACC1409.8390956E-810.1651283
Produced by FastQC (version 0.11.5)