FastQCFastQC Report
Thu 26 May 2016
SRR1512638_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512638_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences755199
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT52350.6931947738278256No Hit
GGTATCAACGCAGAGTACTTTTTTT41690.5520399259003256No Hit
TATCAACGCAGAGTACTTTTTTTTT39560.5238354394007407No Hit
ACGCAGAGTACTTTTTTTTTTTTTT17750.23503738749654063No Hit
GTACTTTTTTTTTTTTTTTTTTTTT13810.1828657082437874No Hit
GAGTACTTTTTTTTTTTTTTTTTTT11200.14830528112457778No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA8150.014.5950791
TAGGACG1650.012.6745324
GTATTAA853.8623893E-612.3148141
TCCTATA701.08183085E-412.2234912
TAGCACC550.00305170812.0984174
CTCCTAT657.9027103E-411.7119891
TTAAGAC1001.9107356E-611.407083
GTTCTAG855.2239244E-511.1952851
GCTTAAT855.2239244E-511.1952851
GACGTGA855.3272615E-511.1737837
GGGTTAG600.005797854611.1019911
CGTGAAA951.3611421E-510.99735459
CAAGGAC1408.234565E-910.8638853
TTAGGAC1158.721818E-710.74583
GTATTAT803.7077413E-410.7054911
GTATATG803.7077413E-410.7054911
TCTAGGC803.744288E-410.6941373
CCTGTAC1002.37754E-510.45649053
GTATCAA16700.010.1427951
ATAGGAC750.00263128510.13962653
Produced by FastQC (version 0.11.5)