##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512638_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 755199 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.42303022117349 33.0 31.0 34.0 28.0 34.0 2 31.555419167663093 34.0 31.0 34.0 28.0 34.0 3 31.633969324641583 34.0 31.0 34.0 28.0 34.0 4 35.13023322329611 37.0 35.0 37.0 32.0 37.0 5 34.87698871423294 37.0 35.0 37.0 32.0 37.0 6 34.73962756836278 37.0 35.0 37.0 32.0 37.0 7 34.707997494700074 37.0 35.0 37.0 32.0 37.0 8 34.6994103540921 37.0 35.0 37.0 32.0 37.0 9 36.24744736155636 39.0 37.0 39.0 32.0 39.0 10 36.05325880992957 39.0 37.0 39.0 31.0 39.0 11 36.16364825694949 39.0 37.0 39.0 32.0 39.0 12 36.089781633715084 39.0 37.0 39.0 32.0 39.0 13 36.073291940270046 39.0 37.0 39.0 32.0 39.0 14 37.27531286455623 40.0 38.0 41.0 31.0 41.0 15 37.237125578820944 40.0 38.0 41.0 31.0 41.0 16 37.184024343252574 40.0 38.0 41.0 31.0 41.0 17 37.16284317113767 40.0 38.0 41.0 31.0 41.0 18 37.095836991309575 40.0 38.0 41.0 31.0 41.0 19 37.128397945442195 40.0 38.0 41.0 31.0 41.0 20 37.07858193668159 40.0 38.0 41.0 31.0 41.0 21 37.0379939592081 40.0 38.0 41.0 31.0 41.0 22 36.92161668646278 40.0 37.0 41.0 31.0 41.0 23 36.85545399292107 40.0 37.0 41.0 31.0 41.0 24 36.78826243149157 40.0 37.0 41.0 31.0 41.0 25 36.70420908925992 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5901523395316026 1101 2 0.5003384918483285 1101 3 0.6988411796764531 1101 4 0.4256131323428747 1101 5 0.6740210074830344 1101 6 0.816391138518128 1101 7 0.7534881934598658 1101 8 0.9041438636094199 1101 9 0.8855280066065134 1101 10 0.9125352190353198 1101 11 1.0049557752370433 1101 12 0.9290217724968031 1101 13 0.8962395142448472 1101 14 1.0882026211978513 1101 15 0.9414839574231593 1101 16 1.0187198589808304 1101 17 1.1411149755323038 1101 18 1.1483528906204796 1101 19 1.230526015619887 1101 20 1.1617844676277613 1101 21 1.0207760818720146 1101 22 1.1707152705547301 1101 23 1.3216975622906446 1101 24 1.2021079789524265 1101 25 1.4115825865440783 1102 1 0.5708087078978004 1102 2 0.6111517073415378 1102 3 0.489598130181907 1102 4 0.6107554471726004 1102 5 0.559296358503687 1102 6 0.8486384333076842 1102 7 0.6431901754449001 1102 8 0.6920807858129123 1102 9 0.6039268270754405 1102 10 0.8780525772924577 1102 11 0.8296767805500167 1102 12 0.7228326238436864 1102 13 0.8808005371829921 1102 14 0.9951196557618402 1102 15 1.0627553427790062 1102 16 1.1727215367620971 1102 17 1.1033564235083801 1102 18 1.2253020402904298 1102 19 1.1557325606615336 1102 20 1.0102842091816626 1102 21 1.23929777016059 1102 22 1.2422090890323574 1102 23 1.1463626415615735 1102 24 0.9608481177506931 1102 25 0.7839635954159831 1103 1 0.36391787109903007 1103 2 0.43698322242854104 1103 3 0.4385220071138711 1103 4 0.41730569539173246 1103 5 0.3273312080137103 1103 6 0.4748394261842108 1103 7 0.23155238531740707 1103 8 0.33988938719502926 1103 9 0.39270412927938736 1103 10 0.6013745323317252 1103 11 0.594980839484009 1103 12 0.3621356336432129 1103 13 0.5055829178059383 1103 14 0.6044158994043158 1103 15 0.6202608355300967 1103 16 0.659437821913798 1103 17 0.7088894674427806 1103 18 0.9338707319893444 1103 19 0.8097873133166829 1103 20 0.790771828634746 1103 21 0.8064492180421752 1103 22 0.7510636318606103 1103 23 0.6797121933986929 1103 24 0.9351762535603925 1103 25 0.8928896010265674 1104 1 0.5597667786353995 1104 2 0.5885515159946628 1104 3 0.6914645214710653 1104 4 0.5437597448840279 1104 5 0.521429420509179 1104 6 0.6139074578942356 1104 7 0.5384626928711356 1104 8 0.6075631773450496 1104 9 0.6021545905950134 1104 10 0.7802940691905889 1104 11 0.797005061921034 1104 12 0.5779708947361826 1104 13 0.6442289871214939 1104 14 0.5043225290021311 1104 15 0.4065069563190775 1104 16 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-1.907427760808595 2314 17 -2.111111972545693 2314 18 -2.1347673071416366 2314 19 -2.066634851776584 2314 20 -2.207630066503036 2314 21 -2.1480062051379676 2314 22 -1.9512243968939842 2314 23 -2.1250437214951603 2314 24 -2.1110840822392802 2314 25 -2.1399562538536543 2315 1 -0.6178118360389213 2315 2 -0.752168044849121 2315 3 -0.813679682589683 2315 4 -0.6195334245294575 2315 5 -0.9054657588788473 2315 6 -0.746971482255347 2315 7 -0.9032358046697553 2315 8 -0.9917821057744902 2315 9 -1.2601304529220059 2315 10 -1.0873737137798685 2315 11 -0.8501037949608659 2315 12 -1.1359686742052517 2315 13 -1.0562584411217273 2315 14 -1.5248494079366424 2315 15 -1.4255343103038598 2315 16 -1.5109356443667608 2315 17 -1.299588903754639 2315 18 -1.2100227510091344 2315 19 -0.7815193708639896 2315 20 -1.11948879171932 2315 21 -0.9200337505818794 2315 22 -1.175108861198467 2315 23 -1.0480544722728595 2315 24 -0.8614323979606411 2315 25 -1.1261568807479208 2316 1 -1.5304411962506528 2316 2 -1.3758877695530138 2316 3 -1.4614597223252233 2316 4 -1.4564131330394616 2316 5 -1.3470200840446438 2316 6 -4.336534204038866 2316 7 -2.361158347744201 2316 8 -1.5671478009519078 2316 9 -1.7942146986023175 2316 10 -1.4790324203722491 2316 11 -1.6205856784080908 2316 12 -1.7939361682874235 2316 13 -1.9012365347091489 2316 14 -2.072185556470906 2316 15 -2.086753548758871 2316 16 -2.198990058137909 2316 17 -2.0397447050999986 2316 18 -2.1185575354532062 2316 19 -2.211346093373642 2316 20 -2.2956808938848923 2316 21 -2.511619267712966 2316 22 -2.3820765555431436 2316 23 -2.6166184460571955 2316 24 -2.6500531656143593 2316 25 -2.5864667257389797 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 9482.0 3 1.0 4 182.0 5 1666.0 6 1927.0 7 1476.0 8 1233.0 9 1094.0 10 1024.0 11 882.0 12 911.0 13 894.0 14 892.0 15 912.0 16 937.0 17 969.0 18 1019.0 19 1179.0 20 1404.0 21 1867.0 22 2425.0 23 3063.0 24 3492.0 25 4033.0 26 4695.0 27 5367.0 28 6306.0 29 7489.0 30 9587.0 31 12307.0 32 16150.0 33 20985.0 34 28290.0 35 38583.0 36 57544.0 37 108486.0 38 396446.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 43.413266564639294 19.19047379072364 13.284070202613634 24.112189442023432 2 16.711187276596814 21.301688163256657 38.0140673649582 23.97305719518833 3 18.87423050083488 25.15098669357348 30.58478626163435 25.389996543957288 4 13.477696075510373 17.56466330588296 36.93895900453004 32.01868161407663 5 13.711864361850179 37.48565233181087 34.9530403802364 13.849442926102551 6 31.99945954466638 36.90515141359537 17.424385927496857 13.671003114241396 7 27.444306111096395 32.281926306587906 22.070806071362348 18.20296151095335 8 26.18656897298457 34.57370932685927 20.63739299853115 18.602328701625005 9 26.680953301775588 14.45700632302806 19.93568365257517 38.92635672262118 10 15.793663197433705 28.34806587392619 33.84515336058213 22.01311756805798 11 34.48358533177034 22.46475002152418 23.720834740683344 19.330829906022135 12 22.825433323884933 25.325771082071263 30.985273743075957 20.863521850967842 13 29.37564730469609 21.57858908104586 25.764647296749636 23.281116317508417 14 21.850463304459296 21.559250926012986 27.86421742798155 28.726068341546167 15 24.118415093739987 28.639095095964638 24.2909581103163 22.95153169997908 16 22.957145373095987 27.6108641504487 26.339645727315702 23.09234474913961 17 22.280015042611907 27.525463858006216 27.326837820516214 22.867683278865663 18 22.122775864329213 26.610838990917408 28.787006029018208 22.479379115735167 19 23.221675046445203 26.633527147436876 28.617396128926327 21.527401677191598 20 23.764597145917833 25.86033614980952 28.514471020221162 21.86059568405149 21 23.677997454975444 25.867089336717875 28.32577903307605 22.129134175230636 22 23.587480286496298 26.11398675570351 28.184053612785075 22.114479345015113 23 23.263669575833653 25.938461253259078 28.25228846966164 22.545580701245633 24 23.067297493773165 26.104510201946773 28.34166888462511 22.48652341965495 25 23.148732982962105 26.084912718369598 28.433432777320945 22.332921521347355 >>END_MODULE >>Per sequence GC content warn #GC Content Count 0 499.0 1 499.0 2 533.5 3 568.0 4 568.0 5 568.0 6 1265.5 7 1963.0 8 1963.0 9 1963.0 10 2445.5 11 2928.0 12 2928.0 13 2928.0 14 3748.0 15 4568.0 16 4568.0 17 4568.0 18 7366.0 19 10164.0 20 10164.0 21 10164.0 22 14845.5 23 19527.0 24 19527.0 25 19527.0 26 26747.0 27 33967.0 28 33967.0 29 33967.0 30 41178.5 31 48390.0 32 48390.0 33 48390.0 34 55120.0 35 61850.0 36 61850.0 37 61850.0 38 67334.5 39 72819.0 40 72819.0 41 72819.0 42 79552.0 43 86285.0 44 86285.0 45 86285.0 46 91172.5 47 96060.0 48 96060.0 49 96060.0 50 95984.5 51 95909.0 52 95909.0 53 95909.0 54 88766.0 55 81623.0 56 81623.0 57 81623.0 58 70766.5 59 59910.0 60 59910.0 61 59910.0 62 49850.5 63 39791.0 64 39791.0 65 39791.0 66 30772.5 67 21754.0 68 21754.0 69 21754.0 70 16034.0 71 10314.0 72 10314.0 73 10314.0 74 7265.5 75 4217.0 76 4217.0 77 4217.0 78 2852.0 79 1487.0 80 1487.0 81 1487.0 82 974.5 83 462.0 84 462.0 85 462.0 86 288.5 87 115.0 88 115.0 89 115.0 90 70.0 91 25.0 92 25.0 93 25.0 94 14.0 95 3.0 96 3.0 97 3.0 98 2.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.15267499030056977 2 0.01562502068991087 3 0.0 4 0.0023834777323592854 5 0.09547152472394693 6 0.037076320281144444 7 0.023702361894017338 8 0.02462926990104595 9 0.02396719275316837 10 0.02396719275316837 11 0.03098521052067071 12 0.029263809936189003 13 0.0192002372884498 14 0.010593234366041268 15 0.0033103857393878963 16 0.0023834777323592854 17 0.0014565697253306744 18 0.0023834777323592854 19 0.0010593234366041268 20 0.0 21 0.0 22 7.944925774530952E-4 23 0.0 24 0.0 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 755199.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 71.30132751899207 #Duplication Level Percentage of deduplicated Percentage of total 1 80.66477962340629 57.515058711758094 2 13.378425986842352 19.077990659528822 3 3.493306491396421 7.472321708018316 4 1.2052205559305644 3.4373530236410743 5 0.48792616874055666 1.739489178122871 6 0.24030049059368067 1.02802463896767 7 0.1341011126741576 0.6693111148808957 8 0.09329581793589953 0.5321692536639862 9 0.05397146261204745 0.3463413239142567 >10 0.2170365274822147 2.80970980326214 >50 0.01619050307876723 0.7958670943646896 >100 0.013398297238697454 1.8231947700633526 >500 9.304373038160002E-4 0.41317433221687744 >1k 9.304373038160002E-4 1.64539977322463 >5k 1.8608746076320004E-4 0.6945946143723036 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 5235 0.6931947738278256 No Hit GGTATCAACGCAGAGTACTTTTTTT 4169 0.5520399259003256 No Hit TATCAACGCAGAGTACTTTTTTTTT 3956 0.5238354394007407 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 1775 0.23503738749654063 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 1381 0.1828657082437874 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 1120 0.14830528112457778 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 1.3241542957551585E-4 9 0.0 0.0 0.0 0.0 1.3241542957551585E-4 10 0.0 0.0 0.0 0.0 1.3241542957551585E-4 11 0.0 0.0 0.0 0.0 1.3241542957551585E-4 12 0.0 0.0 0.0 0.0 1.3241542957551585E-4 13 0.0 0.0 0.0 0.0 6.620771478775793E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GGTATCA 815 0.0 14.595079 1 TAGGACG 165 0.0 12.674532 4 GTATTAA 85 3.8623893E-6 12.314814 1 TCCTATA 70 1.08183085E-4 12.223491 2 TAGCACC 55 0.003051708 12.098417 4 CTCCTAT 65 7.9027103E-4 11.711989 1 TTAAGAC 100 1.9107356E-6 11.40708 3 GTTCTAG 85 5.2239244E-5 11.195285 1 GCTTAAT 85 5.2239244E-5 11.195285 1 GACGTGA 85 5.3272615E-5 11.173783 7 GGGTTAG 60 0.0057978546 11.101991 1 CGTGAAA 95 1.3611421E-5 10.9973545 9 CAAGGAC 140 8.234565E-9 10.863885 3 TTAGGAC 115 8.721818E-7 10.7458 3 GTATTAT 80 3.7077413E-4 10.705491 1 GTATATG 80 3.7077413E-4 10.705491 1 TCTAGGC 80 3.744288E-4 10.694137 3 CCTGTAC 100 2.37754E-5 10.4564905 3 GTATCAA 1670 0.0 10.142795 1 ATAGGAC 75 0.002631285 10.1396265 3 >>END_MODULE