FastQCFastQC Report
Thu 26 May 2016
SRR1512637_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512637_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808113
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT72280.25739704919282097No Hit
GGTATCAACGCAGAGTACTTTTTTT44060.15690251781178322No Hit
TATCAACGCAGAGTACTTTTTTTTT40460.1440825208957047No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC7050.012.1120194
GGTATCA16850.011.7688681
TTAGGAC8650.010.8586173
ATAGGAC3000.010.7525883
GTCCTAC14000.010.7286891
AGGACGT12800.010.2289785
GGCGAGG5500.010.16593119
CTAAGAC2800.010.1652633
CGTGAAA7950.010.1151459
GTATCAA33650.010.0942551
GACGTGA7250.010.0766427
GTCCTAT2251.8189894E-1210.0352581
ACCGAGT856.872217E-410.0141028
GGACGTG12700.010.0106956
GTAGGAC16500.010.0050823
TAGGACG13850.09.8645184
TGTAGGA18150.09.82735352
GTTCTAA3800.09.81710151
CCGTCCA1554.3368345E-89.7658179
GTAGAAC5850.09.7308493
Produced by FastQC (version 0.11.5)