FastQCFastQC Report
Thu 26 May 2016
SRR1512637_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512637_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2808113
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT89780.3197164786459804No Hit
TATCAACGCAGAGTACTTTTTTTTT61810.22011222482855924No Hit
GGTATCAACGCAGAGTACTTTTTTT61000.21722772552244157No Hit
ACGCAGAGTACTTTTTTTTTTTTTT28540.10163408666246693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA18150.012.3234321
CTTACAC3200.010.1006743
GTAGGAC14500.010.0310133
GGACGTG12650.09.8380286
GTCTTAC2151.0913936E-119.739231
GTCCTAC12950.09.7016281
GTATCAA36200.09.5967631
TAGGACC5850.09.5877684
AGGACGT12850.09.5436775
TGTAGGA15750.09.5375452
TTAGGAC8100.09.5065163
GGCGAGG5800.09.49466719
TAGGACA6350.09.4316624
GACGTGA6950.09.4302227
GTATTAT3450.09.3799451
TCCAACG2252.7284841E-119.2838418
TGGCGAG12700.09.19578618
CCCGTTT1356.8768168E-69.14545512
TCCTACA14500.09.11395552
TAGGACG14100.09.0345624
Produced by FastQC (version 0.11.5)