FastQCFastQC Report
Thu 26 May 2016
SRR1512636_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512636_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485463
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT70390.28320679084741956No Hit
GTATCAACGCAGAGTACTTTTTTTT67480.27149871070299575No Hit
GTCCTAAAGTGTGTATTTCTCATTT64970.2613999886540254No Hit
CTGTAGGACGTGGAATATGGCAAGA59440.23915061298438156No Hit
CTTTAGGACGTGAAATATGGCGAGG52890.21279737417133146No Hit
GGTATCAACGCAGAGTACTTTTTTT40240.16190142440261635No Hit
TATCAACGCAGAGTACTTTTTTTTT38610.15534329016364357No Hit
GTACATGGGAAGCAGTGGTATCAAC36870.14834258244842108No Hit
GTCCTACAGTGTGCATTTCTCATTT31320.12601273887400455No Hit
CTGAAGGACCTGGAATATGGCGAGA29840.12005811392082683No Hit
CCCATGTACTCTGCGTTGATACCAC27610.11108594253867388No Hit
GTCCTTCAGTGTGCATTTCTCATTT25580.10291845020424767No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2200.014.65067119
TCCAACG3150.014.44256118
TAACGCA500.001515846613.2814454
TAGGACC11950.012.4637584
GTGTAGG4100.011.7271591
CCGTTTC2200.011.64353113
GTAGAAC9000.011.0678713
CGTTTCC2500.011.008409514
AAATGTC9850.010.7871787
GTCGAGG1605.657057E-1010.66482619
ATACACT9350.010.6535654
GATATAC7300.010.6190291
AGTCGTC3300.010.6083428
AATGTCC10450.010.5932518
AGGACGT38900.010.437865
TGTAGGA39800.010.3923062
GGCGAGG16650.010.30541919
GGACGTG38300.010.3041436
AGGACCT25200.010.2020185
TGTCCAC11350.010.19164410
Produced by FastQC (version 0.11.5)