FastQCFastQC Report
Thu 26 May 2016
SRR1512636_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512636_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2485463
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT86200.3468166695702169No Hit
CTGTAGGACGTGGAATATGGCAAGA69850.2810341574185574No Hit
GTCCTACAGTGGACATTTCTAAATT66910.26920537541697465No Hit
GTCCTAAAGTGTGTATTTCTCATTT64020.2575777631773235No Hit
TATCAACGCAGAGTACTTTTTTTTT61870.24892746341426125No Hit
CTTTAGGACGTGAAATATGGCGAGG61040.24558804536619536No Hit
GGTATCAACGCAGAGTACTTTTTTT56800.22852884955438887No Hit
GTACATGGGAAGCAGTGGTATCAAC44050.17723056026181036No Hit
CTGAAGGACCTGGAATATGGCGAGA35620.14331333840012908No Hit
GTCCTACAGTGTGCATTTCTCATTT30000.12070185715900819No Hit
TTTCTAAATTTTCCACCTTTTTCAG29910.12033975158753119No Hit
ACGCAGAGTACTTTTTTTTTTTTTT29020.11675892982514727No Hit
CCCATGTACTCTGCGTTGATACCAC28730.1155921452059435No Hit
ATTTAGAAATGTCCACTGTAGGACG26790.10778675844299432No Hit
GAATATGGCAAGAAAACTGAAAATC25270.10167119768027125No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC9300.013.3878184
CTATACG604.0778556E-412.6724394
ATTCGTT751.48076415E-512.66555310
CAATGCG600.00588118311.0823599
GGCGAGG17200.011.04176219
TAGAAAT10850.010.862094
TAGAACG803.7565408E-410.692374
TAGCGAG803.7874063E-410.68290518
TCCAACG3150.010.55101818
TTAGGAC25150.010.5055683
CCAACGT1451.454282E-810.47825219
GTCTTAT2100.010.4199941
CGGTTTC1856.002665E-1110.26792113
AGGACCT23250.010.2607945
TTCCACC10850.010.2433411
TTTAGAA12600.010.1838242
TGTAGGA38900.010.1646262
CACCTTT10750.010.1592414
GACGTGA21150.010.1506127
GTCTAGG1502.3916073E-810.1481681
Produced by FastQC (version 0.11.5)