##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512636_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2485463 Sequences flagged as poor quality 0 Sequence length 25 %GC 45 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.148693824852753 33.0 31.0 34.0 28.0 34.0 2 31.30735842778589 33.0 31.0 34.0 28.0 34.0 3 31.40203615986237 34.0 31.0 34.0 28.0 34.0 4 34.904058519479065 37.0 35.0 37.0 32.0 37.0 5 34.64081903452194 37.0 35.0 37.0 32.0 37.0 6 34.50121768056897 37.0 35.0 37.0 31.0 37.0 7 34.461143054634086 37.0 35.0 37.0 31.0 37.0 8 34.44169034099482 37.0 35.0 37.0 31.0 37.0 9 35.96268300916167 39.0 37.0 39.0 31.0 39.0 10 35.73507431009836 39.0 37.0 39.0 30.0 39.0 11 35.85253813876932 39.0 37.0 39.0 30.0 39.0 12 35.778737402246584 39.0 37.0 39.0 30.0 39.0 13 35.76583920179057 39.0 37.0 39.0 30.0 39.0 14 36.92104408715801 40.0 37.0 41.0 31.0 41.0 15 36.89581055923987 40.0 37.0 41.0 31.0 41.0 16 36.85030274037473 40.0 37.0 41.0 31.0 41.0 17 36.81562026873866 40.0 37.0 41.0 30.0 41.0 18 36.74433375190055 40.0 37.0 41.0 30.0 41.0 19 36.764967734381884 40.0 37.0 41.0 30.0 41.0 20 36.71666083944923 40.0 37.0 41.0 30.0 41.0 21 36.66695460765258 40.0 37.0 41.0 30.0 41.0 22 36.560304056024975 40.0 37.0 41.0 30.0 41.0 23 36.490597526497076 40.0 37.0 41.0 30.0 41.0 24 36.440336468497016 40.0 37.0 41.0 30.0 41.0 25 36.35656777027057 40.0 37.0 41.0 30.0 41.0 >>END_MODULE >>Per tile sequence quality warn #Tile Base Mean 1101 1 0.5784421114984752 1101 2 0.61721747083077 1101 3 0.5536560609227372 1101 4 0.582244038354581 1101 5 0.6519444291984655 1101 6 0.7963835203024701 1101 7 0.6109649810479567 1101 8 0.6926796772506734 1101 9 0.9037946834473303 1101 10 0.933848207843397 1101 11 0.8924971213056665 1101 12 0.8702091087021202 1101 13 0.8966448603855142 1101 14 0.9254202855826748 1101 15 1.0198243776961888 1101 16 1.072514051932842 1101 17 1.0132844142919666 1101 18 1.0515148250576587 1101 19 1.0773149513333777 1101 20 1.0209829579992373 1101 21 1.070625686365318 1101 22 1.2424436935572132 1101 23 1.038819977198564 1101 24 1.034165762302159 1101 25 1.1618349258106377 1102 1 0.614797691402444 1102 2 0.6115429840272526 1102 3 0.6451602019430034 1102 4 0.5796049032960937 1102 5 0.6717069247674132 1102 6 0.7540867847799291 1102 7 0.7438557999065125 1102 8 0.6471668030281208 1102 9 0.7358374793610949 1102 10 0.9441234165056969 1102 11 0.9576112007745863 1102 12 0.8376950098977005 1102 13 0.8647768896465777 1102 14 0.9369340943545481 1102 15 1.0227084291286275 1102 16 1.079484500838774 1102 17 1.093052710511877 1102 18 1.1923873396398506 1102 19 1.2128051063396654 1102 20 1.2220934604000604 1102 21 1.2428143041990936 1102 22 1.1644640925131 1102 23 1.1995640718136258 1102 24 1.2080591270019738 1102 25 1.2161353028529547 1103 1 0.42536318953022345 1103 2 0.4357342450467989 1103 3 0.45128235307665676 1103 4 0.3481801015742434 1103 5 0.4458393657262292 1103 6 0.5333293475065872 1103 7 0.5923198444859281 1103 8 0.526317966161649 1103 9 0.6585063474803263 1103 10 0.7763918954395663 1103 11 0.6410919036669398 1103 12 0.5958137998850219 1103 13 0.6749831284058985 1103 14 0.7842526956459324 1103 15 0.8379938317290936 1103 16 0.7360844359745471 1103 17 0.7378317298826929 1103 18 0.8746291357892773 1103 19 0.9290162983672587 1103 20 0.8770859420464348 1103 21 0.7124078708470876 1103 22 0.6565180772405199 1103 23 0.7246591568742176 1103 24 0.7919817911527005 1103 25 0.7585634362373881 1104 1 0.3848711736442354 1104 2 0.3978697467262684 1104 3 0.36862757232849575 1104 4 0.30469471492494904 1104 5 0.2123116952812083 1104 6 0.42486709620466456 1104 7 0.32006767370368294 1104 8 0.26564058852995487 1104 9 0.31941244874349195 1104 10 0.42861341475612136 1104 11 0.45119404325431844 1104 12 0.5421602224713311 1104 13 0.31320022900623457 1104 14 0.4341904657314615 1104 15 0.4515697459770607 1104 16 0.45942851436883814 1104 17 0.5657458929927799 1104 18 0.5918825866218285 1104 19 0.5658339160439851 1104 20 0.5524474581839272 1104 21 0.5888022321165138 1104 22 0.5876894380545892 1104 23 0.6155347955553907 1104 24 0.5865133755585745 1104 25 0.5688608525117829 1105 1 0.5657450478807711 1105 2 0.5521477037912632 1105 3 0.5960099863768598 1105 4 0.4986405284379103 1105 5 0.5430479730996112 1105 6 0.7242978126900681 1105 7 0.6803103041043812 1105 8 0.6969047199784271 1105 9 0.797383882810152 1105 10 0.8817056720083087 1105 11 0.8285358660797826 1105 12 0.7302046536462825 1105 13 0.8051138535676117 1105 14 1.0214258120763517 1105 15 1.0647404236827498 1105 16 1.038504242159405 1105 17 1.02920428754841 1105 18 1.0052974825942442 1105 19 0.8886753128293421 1105 20 0.9519788699757399 1105 21 0.9382828769243048 1105 22 0.9856338224881398 1105 23 0.9906974959116539 1105 24 1.1426245910364656 1105 25 0.9734214385461684 1106 1 0.4397367174636635 1106 2 0.40251020882240596 1106 3 0.33556701458173777 1106 4 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1107 17 1.0060902736161808 1107 18 0.9976021081861006 1107 19 0.988717230922532 1107 20 1.0069124900911746 1107 21 0.9075633130693532 1107 22 1.0158041854939057 1107 23 1.0721133430837 1107 24 1.0102286315797144 1107 25 1.0494445353334854 1108 1 0.3450293196125074 1108 2 0.49251911032682827 1108 3 0.48816203838149974 1108 4 0.46893712094367146 1108 5 0.5104039420358646 1108 6 0.6724485039233201 1108 7 0.6378818951051173 1108 8 0.5345104836063044 1108 9 0.6358191149761581 1108 10 0.700459129310417 1108 11 0.7391281878306586 1108 12 0.7044730970396742 1108 13 0.7088497844068016 1108 14 0.875625631927484 1108 15 0.7589323265250343 1108 16 0.8653824304024766 1108 17 0.861602516501037 1108 18 0.8265846382980655 1108 19 0.9306083408410117 1108 20 0.8077852349328083 1108 21 0.8750605350571874 1108 22 0.8176833395733141 1108 23 0.8327340016142841 1108 24 0.8673513576823453 1108 25 0.9180740783631549 1109 1 -0.022201615596863178 1109 2 0.03861505609932436 1109 3 -0.010366478583673455 1109 4 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15 0.17828615460204134 1110 16 0.1156886305060496 1110 17 0.018645948984115535 1110 18 0.10964902788207098 1110 19 -0.048119789348795905 1110 20 0.10219723701288075 1110 21 0.10241150116068809 1110 22 0.18958391705573518 1110 23 0.09508135008472607 1110 24 0.1417389173090271 1110 25 0.1497166658571274 1111 1 -0.13628900392236432 1111 2 -0.174005436660007 1111 3 -0.15519744630946875 1111 4 -0.3035850948349008 1111 5 -0.2806266666671817 1111 6 -0.2286614596864709 1111 7 -0.14562715205995147 1111 8 -0.2936248558510073 1111 9 -0.4751886066667268 1111 10 -0.2438067893523055 1111 11 -0.2976811656580054 1111 12 -0.298875224796447 1111 13 -0.24723322970300643 1111 14 -0.3555245871929955 1111 15 -0.37733122056565094 1111 16 -0.2734618585983881 1111 17 -0.4605283110351266 1111 18 -0.3077767326641023 1111 19 -0.4764156503420409 1111 20 -0.40577560215834296 1111 21 -0.3777008748252797 1111 22 -0.312374745497209 1111 23 -0.4423644786362715 1111 24 -0.5754790408350487 1111 25 -0.49342561349553904 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-0.929106685612588 2315 4 -0.8568583366071962 2315 5 -0.8640308144045505 2315 6 -0.9837655181364013 2315 7 -1.2002558684018965 2315 8 -1.3833693003717826 2315 9 -1.5244389302983166 2315 10 -1.4199060154515522 2315 11 -1.4300758831930551 2315 12 -1.5167841101285688 2315 13 -1.4624358801214896 2315 14 -1.7563805828192685 2315 15 -1.918775257925489 2315 16 -1.7882597456016285 2315 17 -1.8811746259855369 2315 18 -1.6993163408472967 2315 19 -1.5978138304084908 2315 20 -1.6586521889835382 2315 21 -1.6696550250746611 2315 22 -1.6979807598793712 2315 23 -1.6425167615943295 2315 24 -1.645364203644867 2315 25 -1.7160203044423525 2316 1 -1.6875725687080596 2316 2 -1.6853691183013808 2316 3 -1.628133816272296 2316 4 -1.370160089009076 2316 5 -1.6910538795316015 2316 6 -5.035181575590432 2316 7 -2.9034395302143317 2316 8 -2.1644392721036994 2316 9 -2.1907270941434547 2316 10 -1.8967563170765587 2316 11 -2.0207672082876513 2316 12 -2.1664800033474947 2316 13 -2.284422974117078 2316 14 -2.4741387668609818 2316 15 -2.5578189821285306 2316 16 -2.4572639917312955 2316 17 -2.6982238744970743 2316 18 -2.8510873178771163 2316 19 -2.844657996316947 2316 20 -2.98361887908073 2316 21 -3.3040988072876445 2316 22 -3.1545763242692004 2316 23 -3.415577084176988 2316 24 -3.2089829044332987 2316 25 -3.176322880157869 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 37588.0 3 1.0 4 738.0 5 6125.0 6 7439.0 7 5818.0 8 4524.0 9 3967.0 10 3702.0 11 3445.0 12 3243.0 13 3244.0 14 3074.0 15 3175.0 16 3074.0 17 3255.0 18 3545.0 19 4028.0 20 5114.0 21 6660.0 22 8471.0 23 10596.0 24 12648.0 25 14592.0 26 16900.0 27 19161.0 28 22161.0 29 26752.0 30 33600.0 31 43630.0 32 56326.0 33 73723.0 34 98396.0 35 133205.0 36 201475.0 37 395121.0 38 1206947.0 >>END_MODULE >>Per base sequence content warn #Base G A T C 1 41.603085730310454 20.01083051691543 14.017622153564483 24.36846159920963 2 15.178793108790506 21.242383038929546 40.76208442468026 22.816739427599686 3 19.305055034011772 25.49798568717378 30.312782769246617 24.884176509567833 4 13.267425050485379 17.935054416379355 37.64239814305539 31.15512239007988 5 12.93987271939475 38.42547029555452 35.71918696332186 12.91547002172887 6 31.592343972250642 37.39429573286816 17.911625799045776 13.101734495835423 7 27.608024629253276 31.795762319657122 22.44859850695213 18.147614544137472 8 24.83876004806814 37.92626064167585 20.471537818244162 16.76344149201185 9 26.515650598807095 14.825521146689729 20.39067036855642 38.268157885946756 10 15.579259581215466 29.16315533179599 34.23231554927741 21.025269537711132 11 33.961633117948075 21.955732997410145 25.41052322317226 18.672110661469514 12 23.909034638024472 24.573695449719608 32.07665553465453 19.440614377601392 13 29.446748952910763 22.02299894083825 25.903587306799558 22.626664799451426 14 20.55411704822319 23.046014127550333 27.607309629146776 28.792559195079704 15 22.75647628366895 32.277530643250756 23.23913967142195 21.726853401658342 16 21.36204798068702 28.077815219031333 29.02568022934165 21.53445657094 17 21.319926032384167 29.17729535384878 28.232623001739753 21.270155612027303 18 21.220314541931415 27.05999697432035 31.291965211121624 20.42772327262661 19 23.717089606075103 26.505065737301052 28.0522470420528 21.72559761457104 20 23.810713585930337 27.3940729707688 28.530119764502444 20.265093678798422 21 22.523127481680476 26.53316504812182 27.745494501426897 23.19821296877081 22 23.236557595304166 28.49494877560767 27.528157020274502 20.74033660881366 23 21.735427161860787 28.25763248135257 28.478476646001166 21.528463710785477 24 23.286495629383992 26.975588441907377 28.93176399397778 20.806151934730845 25 22.36279518142093 27.51362623382444 28.631687536688332 21.491891048066297 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 262.0 1 262.0 2 536.0 3 810.0 4 810.0 5 810.0 6 2010.0 7 3210.0 8 3210.0 9 3210.0 10 4284.5 11 5359.0 12 5359.0 13 5359.0 14 8021.0 15 10683.0 16 10683.0 17 10683.0 18 19508.0 19 28333.0 20 28333.0 21 28333.0 22 46537.0 23 64741.0 24 64741.0 25 64741.0 26 96829.5 27 128918.0 28 128918.0 29 128918.0 30 168423.0 31 207928.0 32 207928.0 33 207928.0 34 229593.0 35 251258.0 36 251258.0 37 251258.0 38 263782.0 39 276306.0 40 276306.0 41 276306.0 42 290063.0 43 303820.0 44 303820.0 45 303820.0 46 318932.5 47 334045.0 48 334045.0 49 334045.0 50 315941.0 51 297837.0 52 297837.0 53 297837.0 54 260312.0 55 222787.0 56 222787.0 57 222787.0 58 189379.5 59 155972.0 60 155972.0 61 155972.0 62 127864.0 63 99756.0 64 99756.0 65 99756.0 66 76812.0 67 53868.0 68 53868.0 69 53868.0 70 39412.0 71 24956.0 72 24956.0 73 24956.0 74 17404.5 75 9853.0 76 9853.0 77 9853.0 78 6649.5 79 3446.0 80 3446.0 81 3446.0 82 2204.0 83 962.0 84 962.0 85 962.0 86 616.0 87 270.0 88 270.0 89 270.0 90 170.5 91 71.0 92 71.0 93 71.0 94 40.0 95 9.0 96 9.0 97 9.0 98 6.0 99 3.0 100 3.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.14431918720978748 2 0.01544983771635305 3 0.0 4 0.003621055714770246 5 0.08573855253528216 6 0.037699213385996896 7 0.02466341281282401 8 0.026433706717822796 9 0.024381775146119656 10 0.02627277090827745 11 0.0308996754327061 12 0.029652422908729683 13 0.019272063193054977 14 0.009978020191811345 15 0.003500353857611238 16 0.0035405878099975738 17 0.0020519315717031396 18 0.002856610619429861 19 0.0016093580954534429 20 8.046790477267213E-5 21 0.0 22 0.0010460827620447377 23 0.0 24 4.023395238633607E-5 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2485463.0 >>END_MODULE >>Sequence Duplication Levels warn #Total Deduplicated Percentage 64.15113801535293 #Duplication Level Percentage of deduplicated Percentage of total 1 80.99156826337547 51.957012737436784 2 12.53945668693968 16.088408331228152 3 3.3144069035983064 6.378689241353204 4 1.2229919248396737 3.1382529504820815 5 0.6084037580972447 1.951489672737787 6 0.32820245110937163 1.2632736442856651 7 0.2024809762495897 0.9092569537009579 8 0.14500622995446574 0.7441851736715948 9 0.10480168038658605 0.6050832356448708 >10 0.47868094197325545 5.422489993608902 >50 0.032131592184472875 1.4245090649383254 >100 0.024661738193702754 3.2825356098247043 >500 0.003822765556455795 1.6321780416756284 >1k 0.002945409527095866 3.3264286195222508 >5k 4.386780146738524E-4 1.8762067298891003 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 8620 0.3468166695702169 No Hit CTGTAGGACGTGGAATATGGCAAGA 6985 0.2810341574185574 No Hit GTCCTACAGTGGACATTTCTAAATT 6691 0.26920537541697465 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6402 0.2575777631773235 No Hit TATCAACGCAGAGTACTTTTTTTTT 6187 0.24892746341426125 No Hit CTTTAGGACGTGAAATATGGCGAGG 6104 0.24558804536619536 No Hit GGTATCAACGCAGAGTACTTTTTTT 5680 0.22852884955438887 No Hit GTACATGGGAAGCAGTGGTATCAAC 4405 0.17723056026181036 No Hit CTGAAGGACCTGGAATATGGCGAGA 3562 0.14331333840012908 No Hit GTCCTACAGTGTGCATTTCTCATTT 3000 0.12070185715900819 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2991 0.12033975158753119 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 2902 0.11675892982514727 No Hit CCCATGTACTCTGCGTTGATACCAC 2873 0.1155921452059435 No Hit ATTTAGAAATGTCCACTGTAGGACG 2679 0.10778675844299432 No Hit GAATATGGCAAGAAAACTGAAAATC 2527 0.10167119768027125 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 4.023395238633607E-5 0.0 4 0.0 0.0 0.0 4.023395238633607E-5 0.0 5 0.0 0.0 0.0 4.023395238633607E-5 0.0 6 0.0 0.0 0.0 4.023395238633607E-5 0.0 7 0.0 0.0 0.0 8.046790477267213E-5 0.0 8 0.0 0.0 0.0 8.046790477267213E-5 0.0 9 4.023395238633607E-5 0.0 0.0 1.207018571590082E-4 0.0 10 4.023395238633607E-5 0.0 0.0 1.6093580954534427E-4 0.0 11 4.023395238633607E-5 0.0 0.0 1.6093580954534427E-4 0.0 12 4.023395238633607E-5 0.0 0.0 1.6093580954534427E-4 0.0 13 4.023395238633607E-5 0.0 0.0 1.6093580954534427E-4 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 930 0.0 13.387818 4 CTATACG 60 4.0778556E-4 12.672439 4 ATTCGTT 75 1.48076415E-5 12.665553 10 CAATGCG 60 0.005881183 11.082359 9 GGCGAGG 1720 0.0 11.041762 19 TAGAAAT 1085 0.0 10.86209 4 TAGAACG 80 3.7565408E-4 10.69237 4 TAGCGAG 80 3.7874063E-4 10.682905 18 TCCAACG 315 0.0 10.551018 18 TTAGGAC 2515 0.0 10.505568 3 CCAACGT 145 1.454282E-8 10.478252 19 GTCTTAT 210 0.0 10.419994 1 CGGTTTC 185 6.002665E-11 10.267921 13 AGGACCT 2325 0.0 10.260794 5 TTCCACC 1085 0.0 10.24334 11 TTTAGAA 1260 0.0 10.183824 2 TGTAGGA 3890 0.0 10.164626 2 CACCTTT 1075 0.0 10.15924 14 GACGTGA 2115 0.0 10.150612 7 GTCTAGG 150 2.3916073E-8 10.148168 1 >>END_MODULE