FastQCFastQC Report
Thu 26 May 2016
SRR1512635_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512635_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474644
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT82990.3353613691504717No Hit
GTCCTAAAGTGTGTATTTCTCATTT74180.2997602887526448No Hit
CTGTAGGACGTGGAATATGGCAAGA69160.2794745426008751No Hit
CTTTAGGACGTGAAATATGGCGAGG60790.24565149573029496No Hit
GTCCTACAGTGTGCATTTCTCATTT52410.2117880390068228No Hit
GTATCAACGCAGAGTACTTTTTTTT46070.18616819227331285No Hit
CTGTAGGACCTGGAATATGGCGAGA37530.15165817790356917No Hit
CTGAAGGACCTGGAATATGGCGAGA32490.1312916120460155No Hit
GGTATCAACGCAGAGTACTTTTTTT29050.1173906226511773No Hit
GTCCTTCAGTGTGCATTTCTCATTT28290.11431947383138746No Hit
TATCAACGCAGAGTACTTTTTTTTT27710.11197570236365312No Hit
ATTTAGAAATGTCCACTGTAGGACG25780.1041766007555026No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC14550.013.9332844
TCCAACG2350.012.481770518
GTCCTAG4000.012.0879081
GTGTAGG4600.011.8251271
CGGTAGG957.098144E-611.663771
AGGACCT27950.011.6594845
TAGAAAT10950.011.5929354
GGACCTG27800.011.4838576
GTCCTAA23350.011.3890331
CCAACGA1501.891749E-1011.35530619
TGTAGGA45650.011.1643992
CGTACTG600.006001567511.0522185
GTCCTAC42000.010.8647261
GTAGGAC46050.010.8619293
AAGGCCG700.001527334210.8266645
GGCGAGG17000.010.79867519
AATGTCC10450.010.7606998
CTGTAGG44200.010.7113811
TAGGACA12350.010.6622924
GACGTGG21700.010.5647297
Produced by FastQC (version 0.11.5)