FastQCFastQC Report
Thu 26 May 2016
SRR1512635_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512635_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2474644
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT88060.3558491645667013No Hit
CTGTAGGACGTGGAATATGGCAAGA82760.3344319425339564No Hit
GTCCTAAAGTGTGTATTTCTCATTT75660.3057409469806566No Hit
CTTTAGGACGTGAAATATGGCGAGG71220.28779897229662127No Hit
GTCCTACAGTGTGCATTTCTCATTT55670.2249616510496055No Hit
GTATCAACGCAGAGTACTTTTTTTT51610.20855525077546508No Hit
CTGTAGGACCTGGAATATGGCGAGA43760.17683351625526741No Hit
CTGAAGGACCTGGAATATGGCGAGA39060.15784088539604083No Hit
GGTATCAACGCAGAGTACTTTTTTT33640.13593874512859225No Hit
TATCAACGCAGAGTACTTTTTTTTT32720.13222103866253085No Hit
ATTTAGAAATGTCCACTGTAGGACG29490.11916865617842404No Hit
GTCCTTCAGTGTGCATTTCTCATTT28350.1145619329487393No Hit
TTTCTAAATTTTCCACCTTTTTCAG28220.11403660486114366No Hit
GAATATGGCAAGAAAACTGAAAATC26920.10878332398518736No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC16800.013.4618714
CAGACGT550.00306329312.0940874
CGTGCGC550.003070258312.0904210
AGGACCT30200.011.5799055
GGACCGC752.0728145E-411.4011536
GGACCTG29350.011.3299356
TGTAGGA49900.010.988082
TAGGACA13500.010.9806634
AAATGTC10700.010.9212237
TAGAAAT11550.010.8599974
ACCTTTT10900.010.80340915
CTGTAGG47950.010.7944771
GTCCTAA22350.010.7704621
AATGTCC11300.010.7608448
GGCGAGG18200.010.74796819
GTAGGAC50150.010.5920163
CCACCTT10650.010.5241213
AGGACCG1106.0254297E-610.3669895
TCCAACG2850.010.32894818
TTAGGAC26350.010.3139063
Produced by FastQC (version 0.11.5)