FastQCFastQC Report
Thu 26 May 2016
SRR1512634_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512634_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983453
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT74230.3742463269863214No Hit
CTGTAGGACGTGGAATATGGCAAGA67240.33900475584750434No Hit
GTCCTAAAGTGTGTATTTCTCATTT65160.3285179936202169No Hit
CTTTAGGACGTGAAATATGGCGAGG57080.2877809557372925No Hit
GTCCTACAGTGTGCATTTCTCATTT36730.18518210413859063No Hit
GTATCAACGCAGAGTACTTTTTTTT36560.18432501299501425No Hit
CTGAAGGACCTGGAATATGGCGAGA30000.15125137827818458No Hit
CTGTAGGACCTGGAATATGGCGAGA26560.13390788690228606No Hit
GTACATGGGAAGCAGTGGTATCAAC26320.13269787587606058No Hit
GTCCTTCAGTGTGCATTTCTCATTT24820.12513530696215136No Hit
TATCAACGCAGAGTACTTTTTTTTT23660.1192869203353949No Hit
GGTATCAACGCAGAGTACTTTTTTT23330.11762315517433485No Hit
ATTTAGAAATGTCCACTGTAGGACG22760.11474937898704936No Hit
GAATATGGCAAGAAAACTGAAAATC22520.11353936796082388No Hit
TTTCTAAATTTTCCACCTTTTTCAG21870.11026225476479655No Hit
CCCATGTACTCTGCGTTGATACCAC20500.10335510849009279No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2200.013.76452418
TAGGACC9450.013.7367524
CGTAGGA500.001529152913.2658262
AACCGTG550.00312805212.0601477
TAGTACC951.0733838E-611.9688634
TCGCATT658.291297E-411.64690618
GGCGAGG17200.011.44579119
CCAACGT1251.9357685E-811.35774719
CTAGGAC3350.011.3141373
GCCGGTT2350.011.28233911
GTGTAGG3750.011.2557931
GTCTTAG2500.011.2557931
GTCCTAC34350.011.2347271
CCAACGA1355.013135E-911.21752719
CCGTTTC1451.2714736E-911.10874813
AGGACCT19800.011.102435
ACCTTTT8050.011.06803215
ACTGCCG600.00611568311.0241018
AAATGTC8700.010.8917597
ACTGTTC7300.010.8730878
Produced by FastQC (version 0.11.5)