FastQCFastQC Report
Thu 26 May 2016
SRR1512634_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512634_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1983453
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT79470.4006649010589109No Hit
CTGTAGGACGTGGAATATGGCAAGA76800.38720352839215244No Hit
GTCCTAAAGTGTGTATTTCTCATTT71120.3585666007714829No Hit
CTTTAGGACGTGAAATATGGCGAGG66830.33693765367770245No Hit
GTATCAACGCAGAGTACTTTTTTTT47280.23837217216641887No Hit
GTCCTACAGTGTGCATTTCTCATTT39630.19980307070548178No Hit
CTGAAGGACCTGGAATATGGCGAGA36150.1822579108252124No Hit
TATCAACGCAGAGTACTTTTTTTTT32840.16556984208851935No Hit
CTGTAGGACCTGGAATATGGCGAGA31530.15896519857037197No Hit
GGTATCAACGCAGAGTACTTTTTTT31480.15871311293990834No Hit
GTACATGGGAAGCAGTGGTATCAAC31350.15805769030070288No Hit
GTCCTTCAGTGTGCATTTCTCATTT26610.1341599725327497No Hit
ATTTAGAAATGTCCACTGTAGGACG26570.1339583040283788No Hit
TTTCTAAATTTTCCACCTTTTTCAG26190.13204245323685512No Hit
GAATATGGCAAGAAAACTGAAAATC24570.12387487880983317No Hit
CCCATGTACTCTGCGTTGATACCAC22730.11459812760877117No Hit
GGAATATGGCGAGAAAACTGAAAAT21950.11066559177353838No Hit
ATTCCAGGTCCTTCAGTGTGCATTT20100.10133842344638366No Hit
TTCCAGGTCCTTCAGTGTGCATTTC19960.10063258368108545No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCGGT250.00603829718.99692311
ATTTCGC307.737417E-418.99500815
CGTAAAA402.7644812E-416.6227269
CAGTACG350.002172466816.283499
ACGTAAA551.130085E-515.5433298
TTTCGCA400.00528988114.24589616
TAGGACC11900.013.5747084
TGTCCCG655.4416218E-513.1570245
TCCAACG2050.012.04531218
GTCGAGG1251.8409992E-811.39643119
CCACCTT8650.011.31002213
TATGTCG1604.5474735E-1111.27800116
TTAGGAC21750.011.0969333
GGCGAGG17550.011.09339119
GGACGTA600.005878194711.0829366
CATTTCG600.00588813211.08042114
GATATAC8350.011.0556061
TGTAGGA39950.011.0126722
ACCGTGC951.3626717E-510.9984968
ACTGTTC8650.010.7615348
Produced by FastQC (version 0.11.5)