FastQCFastQC Report
Thu 26 May 2016
SRR1512633_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512633_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136796
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47030.22009588187173693No Hit
GTCCTACAGTGGACATTTCTAAATT43620.20413740946725847No Hit
GTCCTAAAGTGTGTATTTCTCATTT40980.1917824630895977No Hit
CTGTAGGACGTGGAATATGGCAAGA40970.19173566405028838No Hit
CTTTAGGACGTGAAATATGGCGAGG34930.16346904430745846No Hit
GGTATCAACGCAGAGTACTTTTTTT30470.14259667277550125No Hit
TATCAACGCAGAGTACTTTTTTTTT28860.1350620274467006No Hit
GTCCTACAGTGTGCATTTCTCATTT22420.10492344613149782No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA2150.014.08414619
TCCAACG3150.013.21599518
GCCGGTT2000.012.78738111
TAGGACC8050.012.2416124
GTATAGG2150.011.66939451
CCGGTTT2200.011.62571212
TGCCGGT2300.011.11946210
GTCTAAG1903.6379788E-1211.0920671
CCAACGT1201.3470708E-711.03991619
CGGTTTC2450.010.82988313
GTCCTAG3000.010.7047251
GTCCTAC22000.010.5374641
GTGCGCC909.805593E-510.52459311
AATACGG1002.4567687E-510.42768914
TGTAGGA25700.010.3974622
GGCGAGG10500.010.27388119
TTAGGAC14300.010.2046013
GACGTGA12700.010.1474477
CTGTAGG23950.010.1404361
TAGGACA6750.010.1071784
Produced by FastQC (version 0.11.5)