FastQCFastQC Report
Thu 26 May 2016
SRR1512633_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512633_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2136796
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT58340.2730255953305791No Hit
CTGTAGGACGTGGAATATGGCAAGA47330.22149985305101658No Hit
GTCCTACAGTGGACATTTCTAAATT47100.2204234751469022No Hit
GTCCTAAAGTGTGTATTTCTCATTT44440.20797493069062278No Hit
CTTTAGGACGTGAAATATGGCGAGG40760.19075288422479264No Hit
GGTATCAACGCAGAGTACTTTTTTT40150.18789814282692405No Hit
TATCAACGCAGAGTACTTTTTTTTT39700.1857921860580046No Hit
GTACATGGGAAGCAGTGGTATCAAC25540.11952474639600598No Hit
GTCCTACAGTGTGCATTTCTCATTT23840.11156890971342141No Hit
CTGAAGGACCTGGAATATGGCGAGA22490.10525103940666307No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATAC400.00527004114.254043
CCAACGA1301.8189894E-1213.87945119
AGAACCG550.00306258512.0943365
TAGGACC8050.011.6865424
TCCAACG2250.011.39602618
TATTGCG600.005891123311.07972916
GGCGAGG10150.011.04022719
GAACCGT700.001491476410.8574246
GTCCTAA13150.010.4167241
GTCCTAT2650.010.4098991
TGTAGGA26050.010.35994152
AGGACCT16350.010.345445
AATCCCG2400.010.28783919
AGGACGT25050.010.2424245
CTGTAGG25550.010.2384961
GAACAGT6000.010.1335956
GGACGTG24700.010.1156466
GTAGGAC25000.010.1108653
GTCCTAC21400.010.0459051
TAGGACA6750.09.8546454
Produced by FastQC (version 0.11.5)