FastQCFastQC Report
Thu 26 May 2016
SRR1512632_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512632_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314846
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT81310.35125446789980846No Hit
GTCCTAAAGTGTGTATTTCTCATTT74110.3201508869272513No Hit
CTGTAGGACGTGGAATATGGCAAGA71780.3100854225291877No Hit
CTTTAGGACGTGAAATATGGCGAGG65240.28183300314578164No Hit
GTATCAACGCAGAGTACTTTTTTTT46260.1998405077486796No Hit
GTCCTACAGTGTGCATTTCTCATTT43620.18843586139207533No Hit
CTGAAGGACCTGGAATATGGCGAGA32760.14152129342513498No Hit
CTGTAGGACCTGGAATATGGCGAGA31450.13586216966485026No Hit
GGTATCAACGCAGAGTACTTTTTTT28740.12415512738212392No Hit
TATCAACGCAGAGTACTTTTTTTTT28400.12268634716953092No Hit
TTTCTAAATTTTCCACCTTTTTCAG27470.11866880129390897No Hit
ATTTAGAAATGTCCACTGTAGGACG26850.11599043737682765No Hit
GTCCTTCAGTGTGCATTTCTCATTT26530.11460805600026958No Hit
GAATATGGCAAGAAAACTGAAAATC23590.10190742710314206No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2000.013.71979618
TAGGACC13500.013.4060664
GGCGAGG19900.011.7472619
AGATCGT658.2017895E-411.6621435
CCAACGA1302.7757778E-911.64848219
AATGTCC10450.011.395058
AGGACCT27050.011.3145425
GGTATCA14550.011.243181
GGACCTG25850.011.1435666
CCACCTT10550.011.13755413
GTCCTAC40300.011.0820361
AAATGTC10650.011.0327377
ACCTTTT11200.010.83289815
TAGAAAT11550.010.8291324
CTAAGAC2450.010.8291323
TGTCGAG2100.010.81363318
ACTCGGA700.001558098810.8007518
CCAACGT1159.2586197E-710.69887719
CACCTTT11000.010.68517614
GTATTAC2450.010.6505451
Produced by FastQC (version 0.11.5)