FastQCFastQC Report
Thu 26 May 2016
SRR1512632_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512632_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2314846
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT84210.3637822991248662No Hit
CTGTAGGACGTGGAATATGGCAAGA80090.3459841389016807No Hit
GTCCTAAAGTGTGTATTTCTCATTT76170.32904996703884404No Hit
CTTTAGGACGTGAAATATGGCGAGG74180.32045328285337343No Hit
GTATCAACGCAGAGTACTTTTTTTT59100.25530856048307315No Hit
GTCCTACAGTGTGCATTTCTCATTT47990.2073140070656968No Hit
TATCAACGCAGAGTACTTTTTTTTT40450.1747416458805467No Hit
CTGAAGGACCTGGAATATGGCGAGA40330.1742232528643374No Hit
GGTATCAACGCAGAGTACTTTTTTT40000.17279767206976188No Hit
CTGTAGGACCTGGAATATGGCGAGA37510.1620410169834192No Hit
ATTTAGAAATGTCCACTGTAGGACG30760.13288140982164687No Hit
TTTCTAAATTTTCCACCTTTTTCAG30510.1318014243712109No Hit
GAATATGGCAAGAAAACTGAAAATC27780.12000798325244963No Hit
GTCCTTCAGTGTGCATTTCTCATTT26320.11370086822190331No Hit
GGAATATGGCGAGAAAACTGAAAAT26050.11253448393543242No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACGCCTA456.767014E-414.77693711
TCCAACG1950.012.6623918
TAGGACC13250.012.2633914
CCAACGA1103.83734E-812.08630419
AGAACGT1052.7124588E-711.765055
GGCGAGG18850.011.43596219
TGTAGGA43950.010.7892372
CCACCTT9950.010.78739813
GGACCTG26050.010.5036446
AGGACGT45300.010.4674785
GGACGTG45150.010.4370836
TTAGGAC26800.010.3887463
AGGACCT27450.010.3506735
CTGTAGG44150.010.2770581
ACCTTTT10350.010.27694415
CACCTTT11300.010.25361714
GACGTGG23400.010.2312937
AATCCCG2700.010.19981819
TGGCGAG45550.010.1743918
TAGAAAT11800.010.1465624
Produced by FastQC (version 0.11.5)