FastQCFastQC Report
Thu 26 May 2016
SRR1512631_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512631_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251034
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT71250.3165212075872688No Hit
GTCCTAAAGTGTGTATTTCTCATTT65590.29137720709682746No Hit
CTGTAGGACGTGGAATATGGCAAGA63610.28258124932808654No Hit
CTTTAGGACGTGAAATATGGCGAGG56750.25210636534143865No Hit
GTATCAACGCAGAGTACTTTTTTTT41930.18626995416328673No Hit
GTCCTACAGTGTGCATTTCTCATTT39010.1732981376558506No Hit
CTGAAGGACCTGGAATATGGCGAGA28540.12678617915144774No Hit
GGTATCAACGCAGAGTACTTTTTTT27960.12420958546161454No Hit
CTGTAGGACCTGGAATATGGCGAGA27510.12221050415053704No Hit
TATCAACGCAGAGTACTTTTTTTTT25570.11359224249833631No Hit
ATTTAGAAATGTCCACTGTAGGACG24230.10763942259423892No Hit
TTTCTAAATTTTCCACCTTTTTCAG24010.1066620939532677No Hit
GTCCTTCAGTGTGCATTTCTCATTT23950.10639554977845736No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCGTGC509.103152E-515.122878
TAGGACC12200.012.8154324
TCCAACG2150.011.88452418
CCAACGT1201.0597432E-811.83029619
GTTAGAC1251.9039362E-811.3707833
GTGTAGG3300.010.9489951
GGCGAGG16850.010.89650919
AGGACCT23200.010.6601095
GGACCTG22650.010.5426836
GTCCTAC35350.010.419871
ACTGTTC6650.010.3756548
GTATAGG2250.010.2596121
GTCCTAA18550.010.2259471
GATATAC5450.010.1286551
TGTAGGA39200.010.07996752
TGGCGAG39150.010.00748818
TTAGACT1901.05501385E-109.9743724
TAGAAAT11250.09.8546784
AAATGTC10300.09.8438597
TCCTACA39600.09.8345782
Produced by FastQC (version 0.11.5)