FastQCFastQC Report
Thu 26 May 2016
SRR1512631_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512631_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2251034
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT76680.34064345540760377No Hit
CTGTAGGACGTGGAATATGGCAAGA73280.325539285501685No Hit
GTCCTAAAGTGTGTATTTCTCATTT68280.3033272709341574No Hit
CTTTAGGACGTGAAATATGGCGAGG66080.2935539845244452No Hit
GTATCAACGCAGAGTACTTTTTTTT53710.23860146048438183No Hit
GTCCTACAGTGTGCATTTCTCATTT42320.18800249129955388No Hit
GGTATCAACGCAGAGTACTTTTTTT35340.1569945189632853No Hit
TATCAACGCAGAGTACTTTTTTTTT34430.1529519323119953No Hit
CTGAAGGACCTGGAATATGGCGAGA32740.14544427138817095No Hit
CTGTAGGACCTGGAATATGGCGAGA31530.14006896386282924No Hit
TTTCTAAATTTTCCACCTTTTTCAG28120.12492036992777542No Hit
ATTTAGAAATGTCCACTGTAGGACG27460.12198838400486177No Hit
GTCCTTCAGTGTGCATTTCTCATTT24850.11039371240061234No Hit
GAATATGGCAAGAAAACTGAAAATC24630.10941638375964113No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC13050.013.5431444
ACCGATC500.001499990813.2999068
ATTTCGC604.1027516E-412.66320115
TGGCGAC905.4367774E-712.66123218
TGGACCG550.00306372712.09378055
CATTTCG658.0428197E-411.68936814
TAATACT3050.011.527074
AGGACCT25000.011.4787265
GGACCTG24450.011.4631076
GGCGAGG16050.011.41852119
GAAATGT9350.011.1773866
TCCAACG2050.011.11717918
AATGTCC8850.011.0564198
AAATGTC9200.010.9463057
CGTGCGC700.001491939510.85706610
CGCATTT700.001497480310.85224219
CACCTTT8650.010.76030114
TTAGGAC24450.010.7262353
GTTTAGG2500.010.6557911
TGTAGGA40150.010.6264332
Produced by FastQC (version 0.11.5)