FastQCFastQC Report
Thu 26 May 2016
SRR1512630_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512630_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338505
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT58930.2519986059469619No Hit
GTCCTAAAGTGTGTATTTCTCATTT52670.22522936662525847No Hit
CTGTAGGACGTGGAATATGGCAAGA49360.2110750244279999No Hit
GTATCAACGCAGAGTACTTTTTTTT48220.20620011503075683No Hit
CTTTAGGACGTGAAATATGGCGAGG45620.19508190061599184No Hit
GTCCTACAGTGTGCATTTCTCATTT32940.14085922416244567No Hit
GGTATCAACGCAGAGTACTTTTTTT30960.1323922762619708No Hit
TATCAACGCAGAGTACTTTTTTTTT29450.12593515942878036No Hit
CTGAAGGACCTGGAATATGGCGAGA23750.1015606124425648No Hit
CTGTAGGACCTGGAATATGGCGAGA23550.10070536517989058No Hit
GTACATGGGAAGCAGTGGTATCAAC23430.10019221682228603No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGAGCAA400.005390918314.20546710
TAGGACC10150.012.9747724
CCAACGT854.101905E-612.25148619
GGACCGT550.00313141612.0585666
GCACCGT752.1269103E-411.3695056
TCCAACG2500.011.35729718
CACCTTT8900.011.07833614
GTCCTAG3050.010.8586411
TGTAGGA32900.010.6550832
CTGTAGG30350.010.5154951
GTCCTAC28500.010.4585861
GGCGAGG14850.010.3914519
CCGGTTT1752.4920155E-1010.28404212
CGGTTTC1951.6370905E-1110.20493813
CCACCTT9350.010.13477413
ACCTTTT9650.010.11908115
AATGTCC7400.010.0889518
GTAGGAC33150.010.0888613
AGGACGT31500.010.0458545
GGTATCA18000.010.0360161
Produced by FastQC (version 0.11.5)