FastQCFastQC Report
Thu 26 May 2016
SRR1512630_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512630_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2338505
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62110.26559703742348206No Hit
GTATCAACGCAGAGTACTTTTTTTT60360.2581136238750826No Hit
CTGTAGGACGTGGAATATGGCAAGA56050.23968304536445295No Hit
GTCCTAAAGTGTGTATTTCTCATTT55970.23934094645938325No Hit
CTTTAGGACGTGAAATATGGCGAGG52560.22475898063078764No Hit
TATCAACGCAGAGTACTTTTTTTTT41170.17605264902149023No Hit
GGTATCAACGCAGAGTACTTTTTTT39800.17019420527217174No Hit
GTCCTACAGTGTGCATTTCTCATTT35360.15120771604080385No Hit
GTACATGGGAAGCAGTGGTATCAAC28660.12255693274121715No Hit
CTGAAGGACCTGGAATATGGCGAGA28240.12076091348960126No Hit
CTGTAGGACCTGGAATATGGCGAGA27940.11947804259558992No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ATCGCGA400.005294372314.244225517
TAGGACC9900.013.342114
CCAACGT1207.4578566E-1012.66099319
AGACCGT701.0913426E-412.21436
TCCAACG2600.012.05280818
GGCGAGG13500.011.39489419
TGCACCG600.005864957311.0864295
AATGTCC7750.011.0315638
GTCCTAC30100.010.969911
GTGTACG700.001471497510.8750691
GGACCGC700.001491875210.8571556
TGTCCAC8850.010.62644910
ATGTCCA8450.010.5673829
ATAGGAC4600.010.535553
ACCTTTT8750.010.52873815
CTAGGAC3250.010.5260163
AAATGTC7900.010.461597
TGTAGGA33900.010.4559462
AGGACCT21200.010.399135
CACCTTT8350.010.35064814
Produced by FastQC (version 0.11.5)