FastQCFastQC Report
Thu 26 May 2016
SRR1512629_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512629_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116450
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67240.3177018119965036No Hit
CTGTAGGACGTGGAATATGGCAAGA61510.2906281745375511No Hit
GTCCTAAAGTGTGTATTTCTCATTT60780.28717900257506673No Hit
CTTTAGGACGTGAAATATGGCGAGG51850.24498570719837465No Hit
GTATCAACGCAGAGTACTTTTTTTT37150.1755297786387583No Hit
GTCCTACAGTGTGCATTTCTCATTT33720.15932339530818115No Hit
CTGAAGGACCTGGAATATGGCGAGA28290.13366722577901677No Hit
CTGTAGGACCTGGAATATGGCGAGA24020.11349193224503296No Hit
GTCCTTCAGTGTGCATTTCTCATTT23260.1099010134895698No Hit
TATCAACGCAGAGTACTTTTTTTTT22340.10555411183821965No Hit
GGTATCAACGCAGAGTACTTTTTTT22230.10503437359729735No Hit
ATTTAGAAATGTCCACTGTAGGACG22070.10427839070141039No Hit
TTTCTAAATTTTCCACCTTTTTCAG22060.10423114177041744No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCTCCGT456.907746E-414.7367666
TAGGACC10100.013.1314594
GTCGAGG1450.013.055319
CCGTTTA550.003126366312.06107913
ATTAGAC1501.8553692E-1011.3683623
AATCCCG2750.011.35811119
GGCGAGG17150.011.25876919
GTACTAG905.4478165E-511.1513421
CTAGGAC3350.011.0290093
TTAGGAC21500.010.9277283
AGGACCT22500.010.9049485
TCCAACG2000.010.88227518
GGACGTG36150.010.7708566
AGGACGT37050.010.713525
TAGACAG3550.010.6742655
GACGTGA20100.010.60481557
GGACCTG22400.010.5732546
TATGTCG2001.8189894E-1210.428925516
ACGTGAA23000.010.3965528
ATAGGAC3100.010.3904393
Produced by FastQC (version 0.11.5)