##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512629_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 2116450 Sequences flagged as poor quality 0 Sequence length 25 %GC 44 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.351813177726854 31.0 26.0 33.0 7.0 34.0 2 27.97264901131612 31.0 27.0 34.0 16.0 34.0 3 29.118637813319474 31.0 28.0 34.0 22.0 34.0 4 32.91264286895509 35.0 33.0 37.0 26.0 37.0 5 33.2109400174821 37.0 35.0 37.0 27.0 37.0 6 33.41796876845661 37.0 35.0 37.0 28.0 37.0 7 33.37946892201564 37.0 35.0 37.0 28.0 37.0 8 33.39139927709135 37.0 35.0 37.0 28.0 37.0 9 34.87634624016631 39.0 35.0 39.0 28.0 39.0 10 34.78775449455456 39.0 35.0 39.0 27.0 39.0 11 34.82565664201847 39.0 35.0 39.0 27.0 39.0 12 34.76330789765881 39.0 35.0 39.0 27.0 39.0 13 34.73226723995369 39.0 35.0 39.0 27.0 39.0 14 35.818855158402044 40.0 36.0 41.0 27.0 41.0 15 35.7485652862104 40.0 36.0 41.0 27.0 41.0 16 35.60723381133502 40.0 36.0 41.0 27.0 41.0 17 35.55702426232607 40.0 36.0 41.0 26.0 41.0 18 35.521438729948734 40.0 36.0 41.0 26.0 41.0 19 35.52901604101207 40.0 36.0 41.0 25.0 41.0 20 35.32642207470056 40.0 36.0 41.0 25.0 41.0 21 35.30200477214203 40.0 36.0 41.0 25.0 41.0 22 35.21611897280824 40.0 36.0 41.0 24.0 41.0 23 35.06462614283352 40.0 36.0 41.0 23.0 41.0 24 34.94732689172908 40.0 36.0 41.0 22.0 41.0 25 34.822154078763965 40.0 36.0 41.0 19.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 0.5340288964220719 1101 2 -1.0126258774611365 1101 3 -1.7310737847271795 1101 4 -2.0194901345617744 1101 5 -2.203108153138558 1101 6 -2.4508319619880155 1101 7 -2.492258293761399 1101 8 -2.406448193025035 1101 9 -2.592187426711014 1101 10 -2.826352738803962 1101 11 -3.0387808958203237 1101 12 -2.7046134493888587 1101 13 -2.926110793798067 1101 14 -3.7353275151815595 1101 15 -3.6971149358671056 1101 16 -3.6424415841142306 1101 17 -3.875855867815929 1101 18 -3.7545723244537577 1101 19 -3.7329005951276173 1101 20 -3.785720112428983 1101 21 -3.784481357318551 1101 22 -4.01509823741841 1101 23 -4.168640854016672 1101 24 -4.406231282747516 1101 25 -4.883942025432294 1102 1 0.6083017331287728 1102 2 -0.15881579375687238 1102 3 -0.8057614101202724 1102 4 -1.540462858356367 1102 5 -1.3527556527419655 1102 6 -1.4054506270123284 1102 7 -1.3921302178396715 1102 8 -1.460886291995763 1102 9 -1.582796125608958 1102 10 -1.7511728726188096 1102 11 -1.8808590851207327 1102 12 -1.756960108217001 1102 13 -2.0579352108076705 1102 14 -2.2590392907010965 1102 15 -2.2685626370326233 1102 16 -2.2825179164304217 1102 17 -2.3899652152586057 1102 18 -2.437893993313345 1102 19 -2.3634759802885412 1102 20 -2.3242485690791312 1102 21 -2.2546242704508828 1102 22 -2.4781482173555176 1102 23 -2.6740051122027992 1102 24 -2.791499285696297 1102 25 -2.891061553627658 1103 1 2.3238141392027067 1103 2 0.5497613616542161 1103 3 -0.16668665236769797 1103 4 -0.396640820468221 1103 5 -0.6099311874961586 1103 6 -0.7037419489993866 1103 7 -0.8504415757926083 1103 8 -0.7940476094262934 1103 9 -0.9323489839232622 1103 10 -1.1995178723377649 1103 11 -1.3244173387148308 1103 12 -1.3352492695408102 1103 13 -1.2550558679044315 1103 14 -1.4028368118383199 1103 15 -1.5059872380573793 1103 16 -1.5324474556869205 1103 17 -1.6724681482991741 1103 18 -1.469922038881812 1103 19 -1.6048531599373277 1103 20 -1.6936400633212827 1103 21 -1.623011773844361 1103 22 -1.6286477810370812 1103 23 -1.7699228140543894 1103 24 -1.9512195396021283 1103 25 -1.9766230211086793 1104 1 1.5919155530302405 1104 2 0.6374191210830311 1104 3 -0.44430291554941803 1104 4 -0.9208286206695746 1104 5 -1.2364849409483654 1104 6 -1.2089356703974516 1104 7 -1.5857540188997028 1104 8 -1.3214560898283736 1104 9 -1.6030964855355805 1104 10 -1.599811538666323 1104 11 -1.6673476921886774 1104 12 -1.7153061181554534 1104 13 -1.7111086321032971 1104 14 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1107 12 -0.43706498661567394 1107 13 -0.4583493417669473 1107 14 -0.43765011232758866 1107 15 -0.6633550360558402 1107 16 -0.6551944812973076 1107 17 -0.8296773272553679 1107 18 -0.7002587407661807 1107 19 -0.7053460469470636 1107 20 -0.5105375502078999 1107 21 -0.5479032083407276 1107 22 -0.7617702251417597 1107 23 -0.8256681766138172 1107 24 -0.8353711236449826 1107 25 -1.0565285881472661 1108 1 -2.615878339199451 1108 2 -4.163581350559465 1108 3 -3.812789925729035 1108 4 -2.9831728803873787 1108 5 -2.058840991728541 1108 6 -1.4493106261809672 1108 7 -1.262137700069374 1108 8 -1.056411087273645 1108 9 -1.3677254601061932 1108 10 -1.2119186015126857 1108 11 -1.213780895820321 1108 12 -1.2353277351031409 1108 13 -1.3002017028889767 1108 14 -1.7234629511741346 1108 15 -1.4420778301157142 1108 16 -1.5047328642626567 1108 17 -1.5542974262574845 1108 18 -1.641242083266377 1108 19 -1.3586983687825338 1108 20 -1.392770205193365 1108 21 -1.4634052905282005 1108 22 -1.4631872912217503 1108 23 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15 0.7441026747726553 2315 16 0.5875515367296131 2315 17 0.7605807292948299 2315 18 0.7408759672224647 2315 19 0.6273493475460796 2315 20 0.7632308162570993 2315 21 0.5738710847818851 2315 22 0.6486199464843665 2315 23 0.8018475660704638 2315 24 0.8164068736386341 2315 25 0.9868100956408554 2316 1 0.456878584242407 2316 2 0.41081555390593394 2316 3 -0.20574995656577855 2316 4 0.03516460404599542 2316 5 -0.7443414957906853 2316 6 -0.41030451813376345 2316 7 -0.29549890507441745 2316 8 -0.1273735494689845 2316 9 -0.1703907628399861 2316 10 -0.110902175016804 2316 11 -0.2109107066487681 2316 12 -0.0010094050313895764 2316 13 -0.08475397709878507 2316 14 -0.1316489204559943 2316 15 -0.10055823099337857 2316 16 -0.07446096720993722 2316 17 -0.03730400871612716 2316 18 0.06896695427143129 2316 19 0.06120526639507062 2316 20 0.06545638556747235 2316 21 0.029867258840241107 2316 22 -0.08597001078528876 2316 23 -0.04278585448260941 2316 24 -0.13203269920450822 2316 25 0.0016557753318480195 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 93595.0 3 9.0 4 794.0 5 3444.0 6 4118.0 7 3894.0 8 3429.0 9 3317.0 10 3346.0 11 3236.0 12 3301.0 13 3480.0 14 3481.0 15 3569.0 16 3592.0 17 3841.0 18 4215.0 19 5130.0 20 6582.0 21 8206.0 22 9836.0 23 11656.0 24 13213.0 25 15336.0 26 17615.0 27 20174.0 28 22684.0 29 27519.0 30 34067.0 31 42815.0 32 56706.0 33 75064.0 34 103879.0 35 153084.0 36 246288.0 37 317728.0 38 784207.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 42.37935264280426 20.2679121278761 12.543939578119772 24.80879565119987 2 13.7761011268622 20.123993670100887 43.269557674150356 22.83034752888656 3 18.49703155921258 25.008379808921422 29.92306672600392 26.571521905862078 4 11.932341878966191 17.29187624415057 39.15559900302265 31.620182873860585 5 12.576357419217436 38.42204526899914 36.527846468757765 12.473750843025663 6 31.190448004318572 37.765708089269395 18.519232317301274 12.524611589110762 7 27.429333065083995 31.65373818738102 22.903242337337463 18.013686410197526 8 24.002650247015154 38.7892939878445 20.930932439554272 16.277123325586068 9 26.309938016186372 15.20410604277049 20.892737448692227 37.593218492350914 10 14.818182377847833 29.246053676534313 35.500928421342756 20.434835524275105 11 33.522593550030756 22.02325959130211 26.25064461335274 18.20350224531439 12 23.71906803079664 24.467056810491357 32.85159711280309 18.962278045908914 13 29.16236623262084 22.582856081859738 25.92921347409864 22.325564211420783 14 19.905429020368004 23.487463565966387 28.105733424853636 28.501373988811974 15 22.078120340427066 33.36279111885695 23.24963624217903 21.309452298536957 16 20.81416051925577 28.300996994247324 30.099746916479585 20.78509557001732 17 20.738134161134326 29.715772705554695 29.000929946264492 20.545163187046487 18 20.592812187741224 27.650866865880857 32.02502332321477 19.73129762316315 19 23.3308335605854 26.955362012544313 27.991820819319457 21.72198360755083 20 23.285646120550094 28.134556140639884 28.941508608692196 19.63828913011783 21 21.939791634507113 27.237570944368628 27.622538551214898 23.20009886990936 22 22.64267169955084 29.589217522662903 27.628724020760174 20.13938675702608 23 21.12474294349112 29.017277227160314 28.782943945585444 21.075035883763118 24 22.91572952488393 27.233415256707723 29.594277845849753 20.256577372558592 25 22.04468374071025 28.18715690998001 28.95442664370232 20.813732705607425 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1680.0 1 1680.0 2 1560.0 3 1440.0 4 1440.0 5 1440.0 6 2129.0 7 2818.0 8 2818.0 9 2818.0 10 3827.5 11 4837.0 12 4837.0 13 4837.0 14 7854.5 15 10872.0 16 10872.0 17 10872.0 18 19329.0 19 27786.0 20 27786.0 21 27786.0 22 45012.0 23 62238.0 24 62238.0 25 62238.0 26 91531.0 27 120824.0 28 120824.0 29 120824.0 30 156960.0 31 193096.0 32 193096.0 33 193096.0 34 212910.0 35 232724.0 36 232724.0 37 232724.0 38 243840.0 39 254956.0 40 254956.0 41 254956.0 42 262792.0 43 270628.0 44 270628.0 45 270628.0 46 272003.5 47 273379.0 48 273379.0 49 273379.0 50 254387.0 51 235395.0 52 235395.0 53 235395.0 54 202257.5 55 169120.0 56 169120.0 57 169120.0 58 142999.5 59 116879.0 60 116879.0 61 116879.0 62 94058.5 63 71238.0 64 71238.0 65 71238.0 66 54582.0 67 37926.0 68 37926.0 69 37926.0 70 27451.0 71 16976.0 72 16976.0 73 16976.0 74 11880.5 75 6785.0 76 6785.0 77 6785.0 78 4756.5 79 2728.0 80 2728.0 81 2728.0 82 1863.5 83 999.0 84 999.0 85 999.0 86 756.5 87 514.0 88 514.0 89 514.0 90 406.5 91 299.0 92 299.0 93 299.0 94 240.5 95 182.0 96 182.0 97 182.0 98 156.5 99 131.0 100 131.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.936733681400459 2 0.21583311677573297 3 0.19986297810012046 4 0.192066904486286 5 0.1665524817501004 6 0.21923503980722436 7 0.4232559238347232 8 0.3051335963523826 9 0.25585296132674995 10 0.22684211769708712 11 0.22296770535566632 12 0.2325119894162395 13 0.24706466016206383 14 0.2571759313945522 15 0.3409955349760212 16 0.5112806822745636 17 0.5174702922346383 18 0.22773984738595288 19 0.20118594816792268 20 0.18587729452621135 21 0.21658909967161993 22 0.28647026861017266 23 0.19249214486522243 24 0.3191665288572846 25 0.36258829643979307 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 2116450.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.28388023854835 #Duplication Level Percentage of deduplicated Percentage of total 1 87.1312274146804 63.85314434895159 2 9.047421486828998 13.260603054168907 3 2.050033989168269 4.507033360414828 4 0.7112202009323096 2.0848390411343867 5 0.3034287070872474 1.1118216515559702 6 0.17534589697367398 0.7710016628483737 7 0.10593893930299216 0.5434531578335523 8 0.07451734233589015 0.43687359931506264 9 0.05880535853601813 0.3878536367104604 >10 0.2947031546065863 3.9024682483904596 >50 0.02240752123226051 1.1199719491231872 >100 0.019949788370404304 2.960006116358625 >500 0.002532568803275224 1.2507298448654813 >1k 0.0022078804951627495 2.6615009525068607 >5k 2.597506464897352E-4 1.1486993758222432 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTCCTACAGTGGACATTTCTAAATT 6724 0.3177018119965036 No Hit CTGTAGGACGTGGAATATGGCAAGA 6151 0.2906281745375511 No Hit GTCCTAAAGTGTGTATTTCTCATTT 6078 0.28717900257506673 No Hit CTTTAGGACGTGAAATATGGCGAGG 5185 0.24498570719837465 No Hit GTATCAACGCAGAGTACTTTTTTTT 3715 0.1755297786387583 No Hit GTCCTACAGTGTGCATTTCTCATTT 3372 0.15932339530818115 No Hit CTGAAGGACCTGGAATATGGCGAGA 2829 0.13366722577901677 No Hit CTGTAGGACCTGGAATATGGCGAGA 2402 0.11349193224503296 No Hit GTCCTTCAGTGTGCATTTCTCATTT 2326 0.1099010134895698 No Hit TATCAACGCAGAGTACTTTTTTTTT 2234 0.10555411183821965 No Hit GGTATCAACGCAGAGTACTTTTTTT 2223 0.10503437359729735 No Hit ATTTAGAAATGTCCACTGTAGGACG 2207 0.10427839070141039 No Hit TTTCTAAATTTTCCACCTTTTTCAG 2206 0.10423114177041744 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 4.7248930992936286E-5 0.0 9 0.0 0.0 0.0 4.7248930992936286E-5 0.0 10 0.0 0.0 0.0 4.7248930992936286E-5 0.0 11 0.0 0.0 0.0 1.4174679297880886E-4 0.0 12 0.0 4.7248930992936286E-5 0.0 1.4174679297880886E-4 0.0 13 0.0 4.7248930992936286E-5 0.0 1.4174679297880886E-4 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position GCTCCGT 45 6.907746E-4 14.736766 6 TAGGACC 1010 0.0 13.131459 4 GTCGAGG 145 0.0 13.0553 19 CCGTTTA 55 0.0031263663 12.061079 13 ATTAGAC 150 1.8553692E-10 11.368362 3 AATCCCG 275 0.0 11.358111 19 GGCGAGG 1715 0.0 11.258769 19 GTACTAG 90 5.4478165E-5 11.151342 1 CTAGGAC 335 0.0 11.029009 3 TTAGGAC 2150 0.0 10.927728 3 AGGACCT 2250 0.0 10.904948 5 TCCAACG 200 0.0 10.882275 18 GGACGTG 3615 0.0 10.770856 6 AGGACGT 3705 0.0 10.71352 5 TAGACAG 355 0.0 10.674265 5 GACGTGA 2010 0.0 10.6048155 7 GGACCTG 2240 0.0 10.573254 6 TATGTCG 200 1.8189894E-12 10.4289255 16 ACGTGAA 2300 0.0 10.396552 8 ATAGGAC 310 0.0 10.390439 3 >>END_MODULE