FastQCFastQC Report
Thu 26 May 2016
SRR1512629_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512629_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2116450
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT72920.3445392048004914No Hit
CTGTAGGACGTGGAATATGGCAAGA69510.3284273193319001No Hit
GTCCTAAAGTGTGTATTTCTCATTT65570.3098112405206832No Hit
CTTTAGGACGTGAAATATGGCGAGG60920.28784048760896785No Hit
GTATCAACGCAGAGTACTTTTTTTT49980.23615015710269555No Hit
GTCCTACAGTGTGCATTTCTCATTT36390.17193885988329513No Hit
CTGAAGGACCTGGAATATGGCGAGA33280.15724444234449197No Hit
TATCAACGCAGAGTACTTTTTTTTT32590.15398426610597934No Hit
GGTATCAACGCAGAGTACTTTTTTT32390.1530392874861206No Hit
CTGTAGGACCTGGAATATGGCGAGA27000.12757211368092797No Hit
GTCCTTCAGTGTGCATTTCTCATTT24830.11731909565546081No Hit
TTTCTAAATTTTCCACCTTTTTCAG24610.11627961917361619No Hit
ATTTAGAAATGTCCACTGTAGGACG24520.11585437879467976No Hit
GAATATGGCAAGAAAACTGAAAATC22570.10664083725105719No Hit
GTACATGGGAAGCAGTGGTATCAAC21500.10158520163481301No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCAACGA1205.2750693E-1113.45229219
TGGCGAC500.001504943413.2940318
ACTGCGG655.4543038E-513.1538468
TAGGACC10500.012.5788664
TATGTCG1750.012.48040516
TGTCGAG1950.012.1740218
GTCGAGG1900.011.99461419
TGCGAGA907.5014123E-611.605919
GGCGAGG13950.011.50379519
TCCAACG1900.011.49483818
CAGTCCG752.0744909E-411.49
CGGACAT855.3260163E-511.17620710
CTAGGAC3250.011.1100563
ACCTTTT8250.011.05114915
TGTAGGA37450.010.9101982
TAGGACT4150.010.7613264
TACAGCG1158.7881745E-710.7421775
GGACGTA1158.814968E-710.7396376
GTACTAG1951.8189894E-1210.7349491
ATGTCGA2050.010.65375217
Produced by FastQC (version 0.11.5)