FastQCFastQC Report
Thu 26 May 2016
SRR1512628_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512628_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398672
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86490.36057451789990463No Hit
CTGTAGGACGTGGAATATGGCAAGA75490.31471580941454275No Hit
GTCCTAAAGTGTGTATTTCTCATTT73740.3074201057918715No Hit
CTTTAGGACGTGAAATATGGCGAGG63930.26652247576992605No Hit
GTCCTACAGTGTGCATTTCTCATTT43820.1826844187116871No Hit
GTATCAACGCAGAGTACTTTTTTTT40880.17042763662559948No Hit
CTGAAGGACCTGGAATATGGCGAGA34470.14370451649912952No Hit
CTGTAGGACCTGGAATATGGCGAGA30360.12657003541959885No Hit
GTCCTTCAGTGTGCATTTCTCATTT28880.12039995464156834No Hit
ATTTAGAAATGTCCACTGTAGGACG28110.117189845047593No Hit
TTTCTAAATTTTCCACCTTTTTCAG26190.10918541593014802No Hit
GTACATGGGAAGCAGTGGTATCAAC24880.10372406064689128No Hit
GGTATCAACGCAGAGTACTTTTTTT24130.10059733052288933No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12750.013.449424
TTTCGCA500.001523242313.27283716
CGGTAGG751.2366651E-412.051691
GGCGAGG20700.011.38437619
TTAGGAC26200.011.1738153
CCAACGA1701.2732926E-1111.13425819
GTCCTAC40650.011.1177941
AGGACCT24700.010.854875
GGACCTG24150.010.59209256
GACGTGA23100.010.5813947
TTTAGGA29400.010.5376732
GGACGTA909.7867814E-510.52671056
TGGCGAG44600.010.52467818
CCCCTAG1155.294878E-610.479731
ATTTAGA10950.010.4558041
CCACCTT10000.010.42340913
CACCTTT10300.010.40104314
CTGTGCG1002.5329671E-510.3989919
AGGACGT46700.010.3463815
GGACGTG45600.010.3050956
Produced by FastQC (version 0.11.5)