FastQCFastQC Report
Thu 26 May 2016
SRR1512628_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512628_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2398672
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT91250.38041883175357033No Hit
CTGTAGGACGTGGAATATGGCAAGA87080.36303421226411947No Hit
GTCCTAAAGTGTGTATTTCTCATTT78710.3281399040802577No Hit
CTTTAGGACGTGAAATATGGCGAGG71840.29949905614439987No Hit
GTATCAACGCAGAGTACTTTTTTTT52080.21712013981069525No Hit
GTCCTACAGTGTGCATTTCTCATTT47680.19877665641655048No Hit
CTGAAGGACCTGGAATATGGCGAGA41740.17401295383445506No Hit
CTGTAGGACCTGGAATATGGCGAGA34540.14399634464403638No Hit
TATCAACGCAGAGTACTTTTTTTTT33470.13953554300046025No Hit
GGTATCAACGCAGAGTACTTTTTTT33350.13903526618061993No Hit
ATTTAGAAATGTCCACTGTAGGACG32330.13478291321197727No Hit
TTTCTAAATTTTCCACCTTTTTCAG30530.12727876091437262No Hit
GTCCTTCAGTGTGCATTTCTCATTT30450.12694524303447907No Hit
GTACATGGGAAGCAGTGGTATCAAC29980.12498582549010454No Hit
GAATATGGCAAGAAAACTGAAAATC27480.11456339174343137No Hit
GGAATATGGCGAGAAAACTGAAAAT26110.10885189805025447No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGCGATC400.005282477514.2490778
GTACCGT500.001499278913.3008046
CTACGCT655.4411503E-513.1573844
CCTACGC604.0841903E-412.67007353
TAGGACC13950.012.1932434
GGCGAGG18850.011.58689519
GGCGTGC1251.8351784E-811.3992618
ATTTCGA600.0058910311.079843515
AAATGTC11350.010.6299327
CACCTTT10650.010.61168114
CATTTCG909.54954E-510.55223214
GGACGTG48400.010.52129656
AGGACGT48700.010.5176415
TGTAGGA47150.010.5001732
TGGCGAG44150.010.45358518
CTGTAGG46750.010.4411231
ACCTTTT10400.010.41018215
AGGACCT28900.010.32500655
ATTTAGA11200.010.2796621
AATGTCC12250.010.1585268
Produced by FastQC (version 0.11.5)