FastQCFastQC Report
Thu 26 May 2016
SRR1512627_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512627_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554066
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT27820.5021062472701808No Hit
GGTATCAACGCAGAGTACTTTTTTT20360.3674652478224616No Hit
TATCAACGCAGAGTACTTTTTTTTT17950.3239686246764826No Hit
ACGCAGAGTACTTTTTTTTTTTTTT7690.13879212945750147No Hit
GTACTTTTTTTTTTTTTTTTTTTTT6880.12417293246652925No Hit
GAGTACTTTTTTTTTTTTTTTTTTT5610.1012514754559926No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTATTAC401.8011981E-417.5776161
GGTATCA4400.016.2079331
ATTAGTT400.00535482814.21711317
AAAGGCG400.00536774314.2119515
GCGTGCG500.001549957213.2392579
CTAGACA550.003126091312.0586264
AATAGTC550.003126091312.0586265
GTTCTAT802.245287E-411.2998961
CCTTACA855.4597516E-511.14561752
GCACATC700.001522330210.8281546
GCCTTAC650.00702996210.8169951
GTATAGC650.00702996210.8169951
GGTCTAC650.00702996210.8169951
GTCTTTA750.001662714210.7139771
ACACCAT1106.2163454E-610.3359656
TGTCCCC750.002710347510.1026110
GTCTAAT800.00284301110.0443531
GCAGTGT951.684279E-49.9732996
AAAGGTG951.684279E-49.9732995
CTTACAC951.684279E-49.9732993
Produced by FastQC (version 0.11.5)