FastQCFastQC Report
Thu 26 May 2016
SRR1512627_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512627_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences554066
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT33930.6123819183996131No Hit
GGTATCAACGCAGAGTACTTTTTTT26260.47395075676904913No Hit
TATCAACGCAGAGTACTTTTTTTTT23440.4230542931708497No Hit
ACGCAGAGTACTTTTTTTTTTTTTT10640.19203488393079524No Hit
GTACTTTTTTTTTTTTTTTTTTTTT7700.13897261337097025No Hit
GAGTACTTTTTTTTTTTTTTTTTTT6900.12453390029346684No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA5100.015.4862481
GTATAAT602.522907E-514.2734691
ATAATAT551.9532579E-413.8209393
TGCTATA500.001495171513.3026552
TATACTG701.08643E-412.2167235
ATATGAG802.8527264E-511.8773713
CTAAGCC657.995715E-411.6946423
TTATACT752.0653888E-411.4022764
GTTTTAT855.2200216E-511.1948781
TTTATAC600.00585885611.0855463
TCTGGAC951.3527111E-511.0021953
GACAGTC750.00264255510.1335267
ATTCTAT750.00264255510.1335266
GTAAGAC951.6361885E-410.0019963
TGGACTG1054.0822906E-59.9543685
TGGACAG1804.130925E-99.5018975
TATGGTG800.0045002879.5018975
TAGGCAG800.0045002879.5018975
AAGGCTG900.00110910449.5018975
TAAGACT800.0045002879.5018974
Produced by FastQC (version 0.11.5)