FastQCFastQC Report
Thu 26 May 2016
SRR1512626_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512626_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260673
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67260.29752202109725734No Hit
GTCCTAAAGTGTGTATTTCTCATTT65800.29106376729407574No Hit
CTGTAGGACGTGGAATATGGCAAGA60010.26545192515680066No Hit
CTTTAGGACGTGAAATATGGCGAGG55970.2475811406603255No Hit
GTATCAACGCAGAGTACTTTTTTTT47760.21126452167120144No Hit
GTCCTACAGTGTGCATTTCTCATTT34430.15229977975585146No Hit
GGTATCAACGCAGAGTACTTTTTTT31440.13907362984385624No Hit
TATCAACGCAGAGTACTTTTTTTTT30910.13672919524407112No Hit
CTGAAGGACCTGGAATATGGCGAGA28940.12801497607128495No Hit
CTGTAGGACCTGGAATATGGCGAGA25760.11394836847257434No Hit
GTCCTTCAGTGTGCATTTCTCATTT23340.10324359162072533No Hit
ATTTAGAAATGTCCACTGTAGGACG22930.10142997240202366No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCATCGC400.005407972314.19867619
ATACCGT552.0053677E-413.7815846
TAGGACC12000.011.8435494
AATGTCC9050.011.2778868
ATAGGAC3250.011.0782733
GTCCTAA20050.011.062551
CCAACGA1303.4464392E-810.92205819
GGCGAGG17100.010.73895919
AGGACGT39200.010.5383415
GGACGTG39300.010.5356356
GGACCGT909.7778364E-510.5275986
AGGACCT22450.010.5088415
GTCCTAC35350.010.4480621
TATGTCG2001.8189894E-1210.42881916
AAATGTC9650.010.4078277
AGTCGTC2550.010.37696558
GACGTGG19000.010.3726857
TCCAACG1659.949872E-1010.32286818
TGTAGGA40650.010.3027432
CCACCTT8100.010.29525613
Produced by FastQC (version 0.11.5)