FastQCFastQC Report
Thu 26 May 2016
SRR1512626_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512626_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2260673
Sequences flagged as poor quality0
Sequence length25
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT73780.3263629901361232No Hit
CTGTAGGACGTGGAATATGGCAAGA69390.30694399411148804No Hit
GTCCTAAAGTGTGTATTTCTCATTT68730.30402450951552923No Hit
CTTTAGGACGTGAAATATGGCGAGG64270.28429587118526206No Hit
GTATCAACGCAGAGTACTTTTTTTT61250.270937017427996No Hit
TATCAACGCAGAGTACTTTTTTTTT42470.18786441028844067No Hit
GGTATCAACGCAGAGTACTTTTTTT41100.1818042680210716No Hit
GTCCTACAGTGTGCATTTCTCATTT37710.16680873350546496No Hit
CTGAAGGACCTGGAATATGGCGAGA36030.15937731817029707No Hit
CTGTAGGACCTGGAATATGGCGAGA29690.13133257220305636No Hit
TTTCTAAATTTTCCACCTTTTTCAG27220.12040662227575594No Hit
ATTTAGAAATGTCCACTGTAGGACG26510.1172659646043457No Hit
GAATATGGCAAGAAAACTGAAAATC23970.1060303723714133No Hit
GTCCTTCAGTGTGCATTTCTCATTT23780.10518991468469788No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC10600.013.3577244
TCCAACG1600.013.0565418
CCAACGA1457.2759576E-1212.44232119
ACCGCCT550.003068999612.0910078
CCACCTT9400.011.31798913
GGCGAGG17650.011.29770819
CACCTTT9650.011.21916614
TTAGGAC24400.011.1385413
TGTAGGA39400.010.8293462
TAGGACA11700.010.80234054
AGGACGT40900.010.7806965
ATTTAGA10000.010.7491221
TGTACCG803.761802E-410.6906635
GGTATCA14700.010.6772931
GGACGTG40500.010.6497056
ATAGGAC3500.010.5888463
GACGTGA23150.010.5469897
TAGAAAT10650.010.5289364
GATATAC5500.010.377271
TAGACTC1659.258656E-1010.3667035
Produced by FastQC (version 0.11.5)