FastQCFastQC Report
Thu 26 May 2016
SRR1512625_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512625_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147441
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT66440.3093915036548152No Hit
CTGTAGGACGTGGAATATGGCAAGA59610.2775862060936715No Hit
GTCCTAAAGTGTGTATTTCTCATTT55780.25975102459159527No Hit
CTTTAGGACGTGAAATATGGCGAGG46140.21486038498845836No Hit
GTATCAACGCAGAGTACTTTTTTTT46120.2147672508813979No Hit
GTCCTACAGTGTGCATTTCTCATTT36430.16964377601060984No Hit
GGTATCAACGCAGAGTACTTTTTTT29390.13686057032533142No Hit
TATCAACGCAGAGTACTTTTTTTTT27740.12917700649284428No Hit
CTGAAGGACCTGGAATATGGCGAGA26160.12181941203506873No Hit
CTGTAGGACCTGGAATATGGCGAGA25130.11702300552145553No Hit
GTCCTTCAGTGTGCATTTCTCATTT22130.10305288946238801No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG2000.013.7211218
TAGGACC10850.013.1870614
GGCGAGG13300.011.52886319
TCTATAC1950.011.1761963
GTCCTAC32550.011.1632851
AGGACCT20800.011.1154575
GGCCGTC600.005991044511.0547177
GCCGGTT1803.6379788E-1211.04954111
GTCCTAA17050.011.0090961
CCGGTTT1855.456968E-1210.75191112
GGACCTG20500.010.7234596
CCAACGA1252.3577559E-710.60086119
CTAGGAC3950.010.5549553
CGGTTTC1909.094947E-1210.47362413
TCCTACA36750.010.3392222
TGTAGGA36900.010.3228712
CCTACAG36100.010.2891543
GTCGAGG1752.5283953E-1010.27634419
ATTTCTA20700.010.25821415
CCAACGT1401.0228541E-710.141129519
Produced by FastQC (version 0.11.5)