FastQCFastQC Report
Thu 26 May 2016
SRR1512625_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512625_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2147441
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA70390.3277854897992541No Hit
GTCCTACAGTGGACATTTCTAAATT69750.32480519837331967No Hit
GTATCAACGCAGAGTACTTTTTTTT61220.2850835017120377No Hit
GTCCTAAAGTGTGTATTTCTCATTT59810.278517547164276No Hit
CTTTAGGACGTGAAATATGGCGAGG55270.2573761048615538No Hit
GGTATCAACGCAGAGTACTTTTTTT41310.19236849813335966No Hit
TATCAACGCAGAGTACTTTTTTTTT39600.18440553197969117No Hit
GTCCTACAGTGTGCATTTCTCATTT39530.1840795626049796No Hit
CTGAAGGACCTGGAATATGGCGAGA31550.14691905388786003No Hit
CTGTAGGACCTGGAATATGGCGAGA29970.13956145943008447No Hit
GTCCTTCAGTGTGCATTTCTCATTT23400.10896690526072661No Hit
ATTTAGAAATGTCCACTGTAGGACG22370.10417049874711343No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11100.014.2961414
CCAACGT1351.8189894E-1213.36478319
GGACCGT801.9998624E-613.062826
TCCAACG2550.013.0340718
AGGACCT21850.012.090075
GGCGAGG13600.011.87003719
GGACCTG22000.011.5298296
GACCGTC752.0740446E-411.4002797
AGACCGT752.0740446E-411.4002796
ACCGTCC1001.9315703E-611.3994838
ATTTAGA9950.011.0942571
GACGTGG16350.011.0400277
GGACGTG32650.010.8823496
TGTAGGA38200.010.87062
AATGGCG700.001496433310.8531116
AGGACGT33300.010.7865615
ATCCCGT1502.1354936E-910.76592710
GTAGGAC37000.010.7092653
CTGTAGG37050.010.6848511
CCCGTTT1701.4006218E-1010.61617612
Produced by FastQC (version 0.11.5)