FastQCFastQC Report
Thu 26 May 2016
SRR1512624_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512624_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879307
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT61870.3292170997074986No Hit
CTGTAGGACGTGGAATATGGCAAGA55510.2953748376396193No Hit
GTCCTAAAGTGTGTATTTCTCATTT52090.27717664011255216No Hit
GTACATGGGAAGCAGTGGTATCAAC46820.24913438836762702No Hit
CTTTAGGACGTGAAATATGGCGAGG46390.24684631090077352No Hit
CCCATGTACTCTGCGTTGATACCAC37160.19773246201924433No Hit
GTATCAACGCAGAGTACTTTTTTTT35800.1904957518915217No Hit
GTCCTACAGTGTGCATTTCTCATTT25630.13638005924524305No Hit
CTGAAGGACCTGGAATATGGCGAGA25140.13377271515510772No Hit
TATCAACGCAGAGTACTTTTTTTTT22510.1197781948345853No Hit
GGTATCAACGCAGAGTACTTTTTTT22120.11770296178325308No Hit
GTCCTTCAGTGTGCATTTCTCATTT21500.11440387334267367No Hit
GAGTACATGGGAAGCAGTGGTATCA20010.10647541886450697No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCTACG454.4475077E-415.6164581
CCGTGTA400.00545237314.1810059
CATTCGT500.001541273613.25187319
TTGCGAG500.001542204613.25081218
AGGACCG802.0619427E-613.0270685
TAGGACC7850.012.9139194
TCGCATT604.2226404E-412.6198218
GTCTTAG2050.012.2428671
TCCAACG1701.8189894E-1211.69189218
GGACCGG658.205999E-411.6612156
GGCGAGG13450.011.33059819
GTCCTAC28750.011.1390931
AAGTCGT2400.011.0538617
TACATCG600.005996411711.0532695
CTGCGTG600.006092544611.0296719
GTCCTAA15050.011.0063791
CCAACGA1553.3287506E-1010.99233719
GTATTAG2200.010.9518011
CGTGCAC700.001530940410.82335110
GTCTTAC1955.456968E-1210.8113941
Produced by FastQC (version 0.11.5)