FastQCFastQC Report
Thu 26 May 2016
SRR1512624_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512624_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1879307
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA65710.34965016359753887No Hit
GTCCTACAGTGGACATTTCTAAATT64310.34220060905429506No Hit
GTACATGGGAAGCAGTGGTATCAAC55240.29393813783485084No Hit
GTCCTAAAGTGTGTATTTCTCATTT54820.2917032714718777No Hit
CTTTAGGACGTGAAATATGGCGAGG51440.27371791836033177No Hit
GTATCAACGCAGAGTACTTTTTTTT47730.25397659882073553No Hit
CCCATGTACTCTGCGTTGATACCAC39680.2111416601970833No Hit
CTGAAGGACCTGGAATATGGCGAGA31270.16639112183373977No Hit
TATCAACGCAGAGTACTTTTTTTTT31130.1656461663794154No Hit
GGTATCAACGCAGAGTACTTTTTTT30750.16362414443196346No Hit
GTCCTACAGTGTGCATTTCTCATTT28040.14920393528039858No Hit
GAGTACATGGGAAGCAGTGGTATCA25750.1370185924918068No Hit
ATTTAGAAATGTCCACTGTAGGACG22280.11855433944533811No Hit
TTTCTAAATTTTCCACCTTTTTCAG22070.11743690626385152No Hit
CTGTAGGACCTGGAATATGGCGAGA21210.11286075133014456No Hit
GTCCTTCAGTGTGCATTTCTCATTT20680.11004056282448796No Hit
GAATATGGCAAGAAAACTGAAAATC19410.10328275263168817No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AGGACCG653.365978E-614.6183335
TGTACCG551.957142E-413.8209715
CCAACGA1153.8016879E-1013.21337419
CCGGTTT1301.4551915E-1113.15264912
GCCGGTT1251.03682396E-1012.919167511
TGCCGGT1403.6379788E-1212.89202610
TAGGACC8800.012.8488414
TCCAACG2000.012.82144418
CCAACGT1001.4487523E-712.34624719
GGCGAGG14350.011.7142119
GACGTGA17600.011.33540257
CGGTTTC1604.5474735E-1111.27962313
AGGACGT34950.011.1195545
TTAGGAC19850.011.0573513
GGACGTG33950.011.053196
CTGTAGG31200.010.8230011
CTCGAAC1158.849893E-710.73615318
TGTAGGA32900.010.6568812
GTCGAGG1803.6379788E-1110.55234819
ACCTTTT7500.010.51097815
Produced by FastQC (version 0.11.5)