FastQCFastQC Report
Thu 26 May 2016
SRR1512623_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512623_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295911
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT40500.17640056604981638No Hit
GTCCTACAGTGGACATTTCTAAATT38680.16847342950140487No Hit
GTCCTAAAGTGTGTATTTCTCATTT33890.1476102514426735No Hit
CTGTAGGACGTGGAATATGGCAAGA32310.14072845158196462No Hit
CTTTAGGACGTGAAATATGGCGAGG26490.1153790369051762No Hit
GTCCTACAGTGTGCATTTCTCATTT24580.10705989909887623No Hit
GGTATCAACGCAGAGTACTTTTTTT24310.10588389532521078No Hit
TATCAACGCAGAGTACTTTTTTTTT23960.10435944598897778No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT500.001530973213.2638816
TAGGACC8500.012.70657354
AACCGTC604.1883072E-412.6322677
TATGTCG855.444268E-511.15222216
TCGACAT600.005988457711.05541113
GGTATCA12900.011.0545471
GTCCTAC22450.010.6911371
CTAGGAC3550.010.6756233
GGCGAGG9200.010.5970119
GTCCTAA11850.010.5933681
TGGCGAG22200.010.48741818
CCTACAC3300.010.3359433
TGTAGGA24400.010.3288842
GTAGGAC22750.010.3283723
AGGACGT20100.010.181235
TTAGGAC12950.010.1696643
ATTTCTA16400.010.11520115
TGGCGAA750.002732496210.09520518
GTATTAG2600.010.0425131
GTCCTAT3100.010.0425121
Produced by FastQC (version 0.11.5)