FastQCFastQC Report
Thu 26 May 2016
SRR1512623_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512623_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2295911
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT50100.21821403355792102No Hit
GTCCTACAGTGGACATTTCTAAATT40710.17731523565155616No Hit
CTGTAGGACGTGGAATATGGCAAGA36850.16050273725767245No Hit
GTCCTAAAGTGTGTATTTCTCATTT34710.15118181845899079No Hit
TATCAACGCAGAGTACTTTTTTTTT32570.14186089966030913No Hit
GGTATCAACGCAGAGTACTTTTTTT31740.13824577694867093No Hit
CTTTAGGACGTGAAATATGGCGAGG31690.1380279984720662No Hit
GTCCTACAGTGTGCATTTCTCATTT26470.11529192551453431No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCCGCG400.005277505614.2510637
TAGGACC7800.013.4016474
GCCGGTT1207.421477E-1012.66623111
CGGTTTC1352.7284841E-1112.66429913
ACCGTAT658.028295E-411.6919058
TTACACG600.005863374611.0868174
TAATACC1855.456968E-1210.7871734
GGCGAGG9000.010.76184219
TGGCGAG22300.010.73084818
TTAGGAC12350.010.6187213
TCCAACG2150.010.60012918
AGGACCT15750.010.5595645
GGCGAGA14250.010.46197619
AAGGGCG1002.3897082E-510.4539685
AGGACGT19850.010.4372115
TAGGACA7450.010.3321064
CTTAGAC1752.3464963E-1010.3175273
GTCCTAC20550.010.2767511
AATCCCG3050.010.27408219
GGACCTG15700.010.2275466
Produced by FastQC (version 0.11.5)