FastQCFastQC Report
Thu 26 May 2016
SRR1512622_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512622_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491503
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT80040.32125187085867446No Hit
CTGTAGGACGTGGAATATGGCAAGA71590.2873365996348389No Hit
GTCCTAAAGTGTGTATTTCTCATTT67120.2693956218395081No Hit
CTTTAGGACGTGAAATATGGCGAGG55880.2242822906494594No Hit
GTCCTACAGTGTGCATTTCTCATTT41220.16544230530727838No Hit
GTATCAACGCAGAGTACTTTTTTTT37320.14978910320396965No Hit
CTGTAGGACCTGGAATATGGCGAGA30210.12125211167716834No Hit
CTGAAGGACCTGGAATATGGCGAGA29840.11976706429813651No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC11800.013.9697944
CCAACGA2300.012.75998219
TCGGACT604.1901408E-412.6316914
TCCAACG3500.012.44019418
GGCGAGG16300.011.73221419
TGTAGGA43150.011.3736022
GCCGGTT2750.011.36462411
CCGGTTT2800.011.16280912
CGGTTTC2900.011.10941913
ACCTTTT9450.011.03657515
CTAGGAC3700.011.0107223
CTGTAGG39750.010.9329131
GTCCTAA19750.010.925881
CGTTTAC700.001516504210.8359114
GTAGGAC43650.010.830923
GTGTAGG4650.010.7920061
TAGGACA11350.010.7675434
ATCCCGT1951.8189894E-1210.68404310
AGGACCT25700.010.6533815
ACCGTGC803.9625712E-410.6307478
Produced by FastQC (version 0.11.5)