FastQCFastQC Report
Thu 26 May 2016
SRR1512622_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512622_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2491503
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT86390.346738494796113No Hit
CTGTAGGACGTGGAATATGGCAAGA84260.3381894382627675No Hit
GTCCTAAAGTGTGTATTTCTCATTT72950.2927951521631722No Hit
CTTTAGGACGTGAAATATGGCGAGG66090.2652615710276086No Hit
GTATCAACGCAGAGTACTTTTTTTT50560.2029297175239203No Hit
GTCCTACAGTGTGCATTTCTCATTT44420.17828595831512142No Hit
CTGAAGGACCTGGAATATGGCGAGA36690.14726050901805054No Hit
CTGTAGGACCTGGAATATGGCGAGA34730.13939377155074667No Hit
GGTATCAACGCAGAGTACTTTTTTT32470.13032294161395752No Hit
TATCAACGCAGAGTACTTTTTTTTT31600.1268310734524502No Hit
GTACATGGGAAGCAGTGGTATCAAC28490.11434864818545272No Hit
TTTCTAAATTTTCCACCTTTTTCAG27340.10973296038575912No Hit
GTCCTTCAGTGTGCATTTCTCATTT25950.10415399861047729No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC12200.013.3191214
TCCAACG3050.013.07678618
TGACGGT550.003070602712.09024410
GGCGAGG18550.011.51811519
GCGCCAC1001.934537E-611.3982313
TGTAGGA42800.011.3672852
GCCGGTT2100.011.30890211
AGGACGT43600.011.2901575
GGACCTG23950.011.2658736
AGGACCT25450.011.2018615
CTAGGAC3750.011.1494113
GGACGTG42700.011.14716
TTAGGAC23450.011.1029483
TAGGACT5050.011.1019664
GTGCGCC951.3618257E-510.99939511
CCAACGA1900.010.99542119
CTGTAGG42850.010.9678521
AAATGTC9450.010.958397
GTCCTAG4100.010.9058481
AGTACCG700.001488139510.8604715
Produced by FastQC (version 0.11.5)