FastQCFastQC Report
Thu 26 May 2016
SRR1512621_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512621_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59990
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC36236.0393398899816635No Hit
CCCATGTACTCTGCGTTGATACCAC24914.152358726454409No Hit
GAGTACATGGGAAGCAGTGGTATCA16482.747124520753459No Hit
CATGTACTCTGCGTTGATACCACTG9641.6069344890815136No Hit
ACGCAGAGTACATGGGAAGCAGTGG9071.5119186531088515No Hit
GTATCAACGCAGAGTACATGGGAAG8931.488581430238373No Hit
TATCAACGCAGAGTACATGGGAAGC8781.4635772628771462No Hit
GCGTTGATACCACTGCTTCCCATGT8701.4502417069511586No Hit
GGTATCAACGCAGAGTACATGGGAA8431.4052342057009501No Hit
GTATCAACGCAGAGTACTTTTTTTT8211.3685614269044841No Hit
GGTATCAACGCAGAGTACTTTTTTT6271.0451741956992833No Hit
ACTCTGCGTTGATACCACTGCTTCC6151.0251708618103017No Hit
TATCAACGCAGAGTACTTTTTTTTT5390.898483080513419No Hit
GTGGTATCAACGCAGAGTACATGGG5290.8818136356059343No Hit
GTACTTTTTTTTTTTTTTTTTTTTT4960.8268044674112351No Hit
GGGAAGCAGTGGTATCAACGCAGAG4700.7834639106517752No Hit
GCAGTGGTATCAACGCAGAGTACAT4060.676779463243874No Hit
GTACTCTGCGTTGATACCACTGCTT3940.6567761293548925No Hit
CAGTGGTATCAACGCAGAGTACATG3660.6101016836139357No Hit
GCTTCCCATGTACTCTGCGTTGATA3580.596766127687948No Hit
CATGGGAAGCAGTGGTATCAACGCA3570.5950991831971996No Hit
ACATGGGAAGCAGTGGTATCAACGC3480.5800966827804634No Hit
GCAGAGTACATGGGAAGCAGTGGTA3440.5734289048174696No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3400.5667611268544757No Hit
ATACCACTGCTTCCCATGTACTCTG3380.5634272378729788No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3050.5084180696782797No Hit
GATACCACTGCTTCCCATGTACTCT2940.4900816802800466No Hit
AAGCAGTGGTATCAACGCAGAGTAC2800.4667444574095682No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2740.4567427904650775No Hit
ATGGGAAGCAGTGGTATCAACGCAG2710.45174195699283215No Hit
CTGCTTCCCATGTACTCTGCGTTGA2700.4500750125020837No Hit
TACCACTGCTTCCCATGTACTCTGC2630.4384064010668444No Hit
NTACATGGGAAGCAGTGGTATCAAC2490.415069178196366No Hit
CCACTGCTTCCCATGTACTCTGCGT2360.3933988998166361No Hit
GTTGATACCACTGCTTCCCATGTAC2340.39006501083513917No Hit
CCATGTACTCTGCGTTGATACCACT2330.3883980663443907No Hit
CTTCCCATGTACTCTGCGTTGATAC2220.37006167694615766No Hit
ACCATGTACTCTGCGTTGATACCAC2120.3533922320386731No Hit
NCCATGTACTCTGCGTTGATACCAC2040.3400566761126855No Hit
GTACATGGTAAGCAGTGGTATCAAC2020.33672278713118853No Hit
GTACATGGGGAGGAAAAAAAAAAAA2010.3350558426404401No Hit
ATCAACGCAGAGTACATGGGAAGCA1670.2783797299549925No Hit
TGATACCACTGCTTCCCATGTACTC1520.25337556259376565No Hit
TTCCCATGTACTCTGCGTTGATACC1510.25170861810301715No Hit
GTGGTATCAACGCAGAGTACTTTTT1360.2267044507417903No Hit
NAGTACATGGGAAGCAGTGGTATCA1330.22170361726954493No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1210.20170028338056342No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1200.20003333888981498No Hit
GGAAGCAGTGGTATCAACGCAGAGT1160.19336556092682114No Hit
AAAAAGTACTCTGCGTTGATACCAC1060.17669611601933655No Hit
ATACATGGGAAGCAGTGGTATCAAC980.1633605600933489No Hit
GGTAAGCAGTGGTATCAACGCAGAG940.15669278213035506No Hit
GCCATGTACTCTGCGTTGATACCAC940.15669278213035506No Hit
CCCCATGTACTCTGCGTTGATACCA910.1516919486581097No Hit
ATCAACGCAGAGTACTTTTTTTTTT850.14169028171361892No Hit
GGTATCAACGCAGAGTACATGGGGA840.14002333722287047No Hit
NATCAACGCAGAGTACATGGGAAGC820.13668944824137358No Hit
TCAACGCAGAGTACATGGGAAGCAG800.13335555925987663No Hit
GAGTACATGGGGAGGAAAAAAAAAA790.1316886147691282No Hit
GAGTACATGGTAAGCAGTGGTATCA780.13002167027837974No Hit
TGGGAAGCAGTGGTATCAACGCAGA750.12502083680613435No Hit
TCCCATGTACTCTGCGTTGATACCA700.11668611435239205No Hit
AAAGTACTCTGCGTTGATACCACTG670.1116852808801467No Hit
GTACATGGGTAAGCAGTGGTATCAA670.1116852808801467No Hit
NTATCAACGCAGAGTACATGGGAAG660.11001833638939823No Hit
AGTGGTATCAACGCAGAGTACATGG660.11001833638939823No Hit
NTATCAACGCAGAGTACTTTTTTTT650.10835139189864977No Hit
NGTATCAACGCAGAGTACATGGGAA640.10668444740790133No Hit
GGTATCAACGCAGAGTACATGGGGG640.10668444740790133No Hit
AACGCAGAGTACATGGGAAGCAGTG630.10501750291715287No Hit
NCGTTGATACCACTGCTTCCCATGT630.10501750291715287No Hit
NATGTACTCTGCGTTGATACCACTG610.10168361393565595No Hit
GTACATGGGGAAAAAAAAAAAAAAA610.10168361393565595No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CATGGGT700.001480196610.8350914
CCATGTA7800.08.8729672
GAAGCAG10050.08.6426019
GGAAGCA10200.08.5083968
AGCAGTG11800.08.18831611
AAGCAGT11700.08.17733810
CAGTGGT11650.08.13794313
CCCATGT7950.08.0940041
AGTGGTA11600.08.09129114
TGCGTTG8850.08.027760512
GTGGTAT11700.08.02213515
TGATACC8650.08.00105717
CTGCGTT8950.07.938064611
CATGTAC8750.07.90961653
TGTACTC9000.07.90058765
TACTCTG9000.07.90058767
TGGTATC11700.07.860071716
GCAGTGG12350.07.823653712
ATACCAC8700.07.81985919
ATGTACT8900.07.77630854
Produced by FastQC (version 0.11.5)