FastQCFastQC Report
Thu 26 May 2016
SRR1512621_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512621_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59990
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTACATGGGAAGCAGTGGTATCAAC41906.98449741623604No Hit
CCCATGTACTCTGCGTTGATACCAC29054.842473745624271No Hit
GAGTACATGGGAAGCAGTGGTATCA20443.407234539089848No Hit
CATGTACTCTGCGTTGATACCACTG11621.9369894982497082No Hit
TATCAACGCAGAGTACATGGGAAGC11601.9336556092682113No Hit
ACGCAGAGTACATGGGAAGCAGTGG11261.876979496582764No Hit
GTATCAACGCAGAGTACTTTTTTTT10791.7986331055175862No Hit
GTATCAACGCAGAGTACATGGGAAG10411.735289214869145No Hit
GGTATCAACGCAGAGTACATGGGAA10271.7119519919986663No Hit
GCGTTGATACCACTGCTTCCCATGT9791.6319386564427405No Hit
GGTATCAACGCAGAGTACTTTTTTT8711.451908651441907No Hit
TATCAACGCAGAGTACTTTTTTTTT8071.3452242040340057No Hit
ACTCTGCGTTGATACCACTGCTTCC7081.1801966994499082No Hit
GTGGTATCAACGCAGAGTACATGGG5710.9518253042173695No Hit
GTACTTTTTTTTTTTTTTTTTTTTT5150.8584764127354559No Hit
GGGAAGCAGTGGTATCAACGCAGAG4890.815135855975996No Hit
CATGGGAAGCAGTGGTATCAACGCA4840.8068011335222537No Hit
CAGTGGTATCAACGCAGAGTACATG4650.775129188198033No Hit
GCAGTGGTATCAACGCAGAGTACAT4530.7551258543090515No Hit
GTACTCTGCGTTGATACCACTGCTT4400.7334555759293215No Hit
GCAGAGTACATGGGAAGCAGTGGTA4200.7001166861143524No Hit
ACATGGGAAGCAGTGGTATCAACGC4120.6867811301883647No Hit
GCTTCCCATGTACTCTGCGTTGATA4050.6751125187531255No Hit
ACGCAGAGTACTTTTTTTTTTTTTT3760.6267711285214203No Hit
AAAAAAAAAAAAAAAAAAAAAAAAA3590.5984330721786965No Hit
ATACCACTGCTTCCCATGTACTCTG3440.5734289048174696No Hit
CTTATACACATCTCCGAGCCCACGA3360.5600933488914819No Hit
AAGCAGTGGTATCAACGCAGAGTAC3250.5417569594932489No Hit
GATACCACTGCTTCCCATGTACTCT3240.5400900150025004No Hit
CTGCTTCCCATGTACTCTGCGTTGA3230.538423070511752No Hit
GTTGATACCACTGCTTCCCATGTAC3160.5267544590765127No Hit
ATGGGAAGCAGTGGTATCAACGCAG3130.5217536256042674No Hit
TACCACTGCTTCCCATGTACTCTGC2870.4784130688448075No Hit
GAGTACTTTTTTTTTTTTTTTTTTT2700.4500750125020837No Hit
CCATGTACTCTGCGTTGATACCACT2570.42840473412235375No Hit
CCACTGCTTCCCATGTACTCTGCGT2540.42340390065010836No Hit
GTACATGGTAAGCAGTGGTATCAAC2440.4067344557426238No Hit
CTTCCCATGTACTCTGCGTTGATAC2340.39006501083513917No Hit
ATCAACGCAGAGTACATGGGAAGCA2290.3817302883813969No Hit
ATACACATCTCCGAGCCCACGAGAC2140.35672612102017004No Hit
TTCCCATGTACTCTGCGTTGATACC2050.3417236206034339No Hit
GGAAGCAGTGGTATCAACGCAGAGT1760.2933822303717286No Hit
TGATACCACTGCTTCCCATGTACTC1760.2933822303717286No Hit
GCAGAGTACTTTTTTTTTTTTTTTT1700.28338056342723783No Hit
GTACATGGGGAGGAAAAAAAAAAAA1630.27171195199199866No Hit
TTTTTTTTTTTTTTTTTTTTTTTTT1610.26837806301050177No Hit
GGTATCAACGCAGAGTACATGGGGA1300.21670278379729954No Hit
AAAAAGTACTCTGCGTTGATACCAC1300.21670278379729954No Hit
ACACATCTCCGAGCCCACGAGACCT1280.21336889481580265No Hit
GTGGTATCAACGCAGAGTACTTTTT1250.20836806134355723No Hit
ACCATGTACTCTGCGTTGATACCAC1160.19336556092682114No Hit
GGTAAGCAGTGGTATCAACGCAGAG1150.19169861643607267No Hit
GTACATGGGTAAGCAGTGGTATCAA1120.1866977829638273No Hit
TGGGAAGCAGTGGTATCAACGCAGA1090.18169694949158194No Hit
AGTGGTATCAACGCAGAGTACATGG1050.1750291715285881No Hit
AACGCAGAGTACATGGGAAGCAGTG940.15669278213035506No Hit
TCAACGCAGAGTACATGGGAAGCAG930.15502583763960662No Hit
AAAGTACTCTGCGTTGATACCACTG880.1466911151858643No Hit
ATCAACGCAGAGTACTTTTTTTTTT880.1466911151858643No Hit
GAGTACATGGTAAGCAGTGGTATCA870.14502417069511586No Hit
CCCCATGTACTCTGCGTTGATACCA860.1433572262043674No Hit
CTCTGCGTTGATACCACTGCTTCCC840.14002333722287047No Hit
GGTATCAACGCAGAGTACATGGGGG820.13668944824137358No Hit
CTGCGTTGATACCACTGCTTCCCAT760.12668778129688282No Hit
GTACATGGAAAGCAGTGGTATCAAC750.12502083680613435No Hit
ACTGCTTCCCATGTACTCTGCGTTG690.11501916986164361No Hit
ACGCAGAGTACATGGTAAGCAGTGG660.11001833638939823No Hit
TCCCATGTACTCTGCGTTGATACCA640.10668444740790133No Hit
GTACATGGGGAAAAAAAAAAAAAAA620.10335055842640439No Hit
TTGATACCACTGCTTCCCATGTACT600.10001666944490749No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTATAC359.633534E-519.0267331
AAAAAGT250.005917787619.0267331
AAAAGTA250.00594646119.0108532
AGTACTC350.002129482316.2950155
AAGTACT400.005181448514.2581394
AAAGTAC400.005181448514.2581393
TTATACA500.00146409513.3075962
TATACAC550.00299681312.0978153
CAGTGGT11600.08.59687313
GAAGCAG11050.08.5950219
GGAAGCA11150.08.5179368
AGTGGTA11850.08.41550414
GTGGTAT12100.08.2416315
TGGTATC12150.08.20771416
AGCAGTG12550.08.02179111
AAGCAGT12450.08.00993810
GCAGTGG12850.07.834511812
GGGAAGC12500.07.5979987
TGCGTTG8400.07.57538512
CTGCGTT8450.07.53056111
Produced by FastQC (version 0.11.5)