##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512621_1.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 59990 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.382663777296216 33.0 31.0 34.0 30.0 34.0 2 31.560160026671113 33.0 31.0 34.0 29.0 34.0 3 31.688098016336056 34.0 31.0 34.0 28.0 34.0 4 35.20641773628938 37.0 35.0 37.0 33.0 37.0 5 34.61183530588431 37.0 35.0 37.0 32.0 37.0 6 34.97026171028505 37.0 35.0 37.0 32.0 37.0 7 34.92488748124688 37.0 35.0 37.0 32.0 37.0 8 34.97566261043507 37.0 35.0 37.0 32.0 37.0 9 36.50541756959493 39.0 37.0 39.0 32.0 39.0 10 36.45594265710952 39.0 37.0 39.0 32.0 39.0 11 36.48511418569762 39.0 37.0 39.0 32.0 39.0 12 36.43402233705618 39.0 37.0 39.0 32.0 39.0 13 36.34784130688448 39.0 37.0 39.0 32.0 39.0 14 37.49903317219537 40.0 38.0 41.0 32.0 41.0 15 37.37117852975496 40.0 38.0 41.0 32.0 41.0 16 37.09574929154859 40.0 37.0 41.0 32.0 41.0 17 37.402667111185195 40.0 38.0 41.0 32.0 41.0 18 37.371011835305886 40.0 38.0 41.0 32.0 41.0 19 37.21220203367228 40.0 37.0 41.0 32.0 41.0 20 37.415119186531086 40.0 38.0 41.0 32.0 41.0 21 37.310451741956996 40.0 38.0 41.0 32.0 41.0 22 37.4073512252042 40.0 38.0 41.0 32.0 41.0 23 37.289381563593935 40.0 38.0 41.0 32.0 41.0 24 37.2639106517753 40.0 38.0 41.0 32.0 41.0 25 37.20866811135189 40.0 38.0 41.0 32.0 41.0 >>END_MODULE >>Per tile sequence quality pass #Tile Base Mean 1101 1 0.8306092766302164 1101 2 0.9556984984926729 1101 3 1.2746139422539073 1101 4 0.9952703926322073 1101 5 0.5091906434473898 1101 6 1.2448863245058774 1101 7 0.4463229042642993 1101 8 0.704952591049441 1101 9 1.4374172370037286 1101 10 0.893704825366811 1101 11 0.7332073107645911 1101 12 1.2073466287920454 1101 13 0.4874328693594734 1101 14 0.7755994021076233 1101 15 0.9096628716002328 1101 16 1.7254851949334622 1101 17 1.5476635875376488 1101 18 1.3281231677263463 1101 19 2.077743280790841 1101 20 1.1556650793653986 1101 21 1.8724512796026644 1101 22 0.44536864428763323 1101 23 0.8167545107437633 1101 24 0.8884548715871361 1101 25 0.32580215223913456 1102 1 0.5028386305027048 1102 2 0.8020827518088893 1102 3 0.6605788545346059 1102 4 0.8985652150541199 1102 5 0.3277614607345072 1102 6 0.7485234661404547 1102 7 0.6213336017396998 1102 8 0.22955678445979544 1102 9 -0.29942486825942893 1102 10 0.8017065369628753 1102 11 0.0759544224462303 1102 12 -0.12769830060462084 1102 13 0.2974457063299454 1102 14 0.6309695347563178 1102 15 0.5788969323618929 1102 16 0.15637950387655053 1102 17 0.44625152078540253 1102 18 0.2951749435072628 1102 19 1.020832711685152 1102 20 1.0974708132122046 1102 21 0.8848603510618034 1102 22 0.7992411303809135 1102 23 1.0358388068498812 1102 24 0.9252541869487132 1102 25 0.46358563533712527 1103 1 -0.6684228565482542 1103 2 0.06022811521741289 1103 3 -0.43967177203180796 1103 4 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1.1509150230022342 1104 17 1.056260148913097 1104 18 1.60521233206061 1104 19 1.8186469193354213 1104 20 1.4811348914405684 1104 21 1.3746503999545254 1104 22 1.3382115071424892 1104 23 1.6300292008677175 1104 24 1.1363557112512694 1104 25 1.068704991103587 1105 1 -0.18788010181195247 1105 2 0.05561982028653745 1105 3 0.4520332970926191 1105 4 0.7596291652521927 1105 5 0.45962337358113814 1105 6 0.9604646093209865 1105 7 0.2976210946655584 1105 8 0.8682098687835094 1105 9 1.0180623982940489 1105 10 0.48733189066972216 1105 11 0.8433567993562505 1105 12 0.7093135839454661 1105 13 0.7864100526796918 1105 14 1.0789038867653744 1105 15 0.8018737291926783 1105 16 1.0488526221561187 1105 17 0.9988830997327725 1105 18 0.9748580221716665 1105 19 0.6552413138356883 1105 20 0.7547996190978878 1105 21 0.3299650482887415 1105 22 0.8088619566401078 1105 23 1.5165971543314924 1105 24 1.6284234003046834 1105 25 0.7239138752918493 1106 1 0.8348013498009514 1106 2 1.0021924009316976 1106 3 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1107 16 1.4123318848289301 1107 17 1.6238830997327725 1107 18 1.6661022618030046 1107 19 1.616070806923247 1107 20 1.5912051490518095 1107 21 1.550587168104407 1107 22 1.839967947423517 1107 23 1.6686708870503821 1107 24 1.7516952897056015 1107 25 1.0130843822042905 1108 1 -0.20400913407001653 1108 2 -0.008896308745722337 1108 3 -0.1124828319396407 1108 4 -0.09520954442522367 1108 5 -0.15327985222531026 1108 6 0.10562589964357016 1108 7 0.32987915918168653 1108 8 0.44885503007382965 1108 9 0.37290110797147236 1108 10 0.3582996326052026 1108 11 0.14980841225947472 1108 12 0.0802813258809536 1108 13 0.7380229559054996 1108 14 0.5627748545073104 1108 15 1.221228567902358 1108 16 0.613368751188375 1108 17 0.8214637448940607 1108 18 0.9425999576555384 1108 19 0.28427357190020075 1108 20 0.6741544578075676 1108 21 0.8460940805468056 1108 22 0.6314426018013961 1108 23 0.7585326382024604 1108 24 0.9348750132079076 1108 25 0.9819783914208813 1109 1 0.21974453161913488 1109 2 0.37624543123472876 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17 0.8509957757891087 2315 18 0.917611316128955 2315 19 0.9410205051123768 2315 20 0.7441226540819841 2315 21 0.9703557797744295 2315 22 0.9833281083893155 2315 23 1.0056930198471647 2315 24 1.0530031367880994 2315 25 0.8184163741560013 2316 1 -0.5655219260611162 2316 2 -0.4471610473441636 2316 3 -0.6909032991254023 2316 4 -0.47711536570813706 2316 5 -0.3727830038752913 2316 6 -0.7475442894554334 2316 7 -0.29155206069224704 2316 8 -0.36148979751237675 2316 9 -0.9418931078238586 2316 10 -0.9792976977618721 2316 11 -0.8266469084672607 2316 12 -0.5947279436778174 2316 13 -0.8072124908872382 2316 14 -1.1433801325153112 2316 15 -1.2492942352122185 2316 16 -0.8895975076177081 2316 17 -1.455139888772976 2316 18 -0.9825020074909219 2316 19 -1.1860638400389902 2316 20 -1.7355600397823494 2316 21 -1.1659563458528979 2316 22 -1.1366330377981555 2316 23 -0.736091017711523 2316 24 -1.1830337743338077 2316 25 -1.4347809708334722 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 727.0 3 0.0 4 23.0 5 128.0 6 141.0 7 99.0 8 83.0 9 78.0 10 82.0 11 78.0 12 46.0 13 65.0 14 62.0 15 57.0 16 51.0 17 60.0 18 65.0 19 69.0 20 117.0 21 115.0 22 157.0 23 190.0 24 240.0 25 289.0 26 327.0 27 374.0 28 413.0 29 538.0 30 648.0 31 883.0 32 1084.0 33 1512.0 34 2056.0 35 2880.0 36 4581.0 37 10018.0 38 31654.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 49.290578887627696 18.019296254256524 11.759698203912667 20.93042665420311 2 12.746307471743407 24.238989097456074 36.835261561030904 26.179441869769615 3 22.94087249329044 32.76934104585841 27.29500408408208 16.994782376769074 4 13.730099191464532 20.64516129032258 28.810535967325162 36.81420355088772 5 10.408016940956765 43.316158938188856 32.943157754322776 13.332666366531607 6 30.53818315238238 29.02386551258318 20.765164023281798 19.67278731175264 7 23.452795890799326 35.51023130930741 21.209746010039524 19.827226789853743 8 32.14279759224984 29.468260717322796 22.009904457005653 16.379037233421705 9 32.06103051525763 13.211605802901449 18.82441220610305 35.90295147573787 10 20.57460148069099 32.119989328353235 27.824651504035213 19.480757686920562 11 27.789821237993596 27.538020277481323 17.11746264674493 27.55469583778015 12 27.974989578991245 32.930387661525636 24.985410587744894 14.109212171738225 13 32.60126402854904 20.52795704304034 22.09215068287558 24.778628245535046 14 24.17391884231936 29.255443299656562 24.919142409389483 21.65149544863459 15 37.348493689668395 20.29309281272403 22.523799203080976 19.834614294526602 16 16.975910644327747 27.526881720430108 34.77369342335584 20.723514211886304 17 26.876271129930316 30.44377021304971 25.99106458173574 16.68889407528423 18 26.47828623822622 21.562057180961908 31.93465032924898 20.02500625156289 19 22.392438613745856 30.09385053926553 33.91955191785161 13.59415892913701 20 24.682447074512417 25.10251708618103 29.608268044674112 20.60676779463244 21 19.476579429904984 27.09284880813469 35.25754292382064 18.17302883813969 22 21.518354282665957 29.08345280565465 23.41713066382156 25.981062247857835 23 17.12675868507035 28.890778155631125 31.237914249516567 22.744548909781955 24 20.730467252329593 36.204970911333746 25.58302355431829 17.48153828201837 25 20.575095849308216 25.21086847807968 24.437406234372393 29.776629438239706 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 536.0 1 536.0 2 380.0 3 224.0 4 224.0 5 224.0 6 411.0 7 598.0 8 598.0 9 598.0 10 482.5 11 367.0 12 367.0 13 367.0 14 280.5 15 194.0 16 194.0 17 194.0 18 378.5 19 563.0 20 563.0 21 563.0 22 879.0 23 1195.0 24 1195.0 25 1195.0 26 1708.0 27 2221.0 28 2221.0 29 2221.0 30 2497.5 31 2774.0 32 2774.0 33 2774.0 34 2761.5 35 2749.0 36 2749.0 37 2749.0 38 2671.5 39 2594.0 40 2594.0 41 2594.0 42 3236.0 43 3878.0 44 3878.0 45 3878.0 46 11696.0 47 19514.0 48 19514.0 49 19514.0 50 16142.0 51 12770.0 52 12770.0 53 12770.0 54 8863.5 55 4957.0 56 4957.0 57 4957.0 58 3501.0 59 2045.0 60 2045.0 61 2045.0 62 1734.0 63 1423.0 64 1423.0 65 1423.0 66 1096.0 67 769.0 68 769.0 69 769.0 70 552.5 71 336.0 72 336.0 73 336.0 74 262.0 75 188.0 76 188.0 77 188.0 78 126.5 79 65.0 80 65.0 81 65.0 82 42.0 83 19.0 84 19.0 85 19.0 86 12.5 87 6.0 88 6.0 89 6.0 90 4.5 91 3.0 92 3.0 93 3.0 94 2.5 95 2.0 96 2.0 97 2.0 98 1.0 99 0.0 100 0.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.13668944824137358 2 0.006667777962993833 3 0.0016669444907484582 4 0.00833472245374229 5 0.028338056342723787 6 0.048341390231705285 7 0.045007501250208366 8 0.028338056342723787 9 0.03333888981496916 10 0.030005000833472247 11 0.03667277879646608 12 0.025004167361226872 13 0.038339723287214536 14 0.013335555925987666 15 0.015002500416736123 16 0.00833472245374229 17 0.006667777962993833 18 0.00833472245374229 19 0.0016669444907484582 20 0.0 21 0.0 22 0.006667777962993833 23 0.0033338889814969164 24 0.0016669444907484582 25 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 59990.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 30.880146691115186 #Duplication Level Percentage of deduplicated Percentage of total 1 81.75978407557355 25.247541256876143 2 10.909581646423753 6.737789631605268 3 3.071524966261808 2.845474245707618 4 1.2901484480431848 1.593598933155526 5 0.6207827260458839 0.9584930821803634 6 0.3508771929824561 0.6501083513918987 7 0.25371120107962214 0.5484247374562428 8 0.19973009446693657 0.4934155692615436 9 0.11336032388663966 0.31505250875145857 >10 0.9986504723346828 6.23770628438073 >50 0.1349527665317139 2.8988164694115683 >100 0.21592442645074222 17.82630438406401 >500 0.032388663967611336 7.419569928321387 >1k 0.048582995951417005 26.22770461743624 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GTACATGGGAAGCAGTGGTATCAAC 4190 6.98449741623604 No Hit CCCATGTACTCTGCGTTGATACCAC 2905 4.842473745624271 No Hit GAGTACATGGGAAGCAGTGGTATCA 2044 3.407234539089848 No Hit CATGTACTCTGCGTTGATACCACTG 1162 1.9369894982497082 No Hit TATCAACGCAGAGTACATGGGAAGC 1160 1.9336556092682113 No Hit ACGCAGAGTACATGGGAAGCAGTGG 1126 1.876979496582764 No Hit GTATCAACGCAGAGTACTTTTTTTT 1079 1.7986331055175862 No Hit GTATCAACGCAGAGTACATGGGAAG 1041 1.735289214869145 No Hit GGTATCAACGCAGAGTACATGGGAA 1027 1.7119519919986663 No Hit GCGTTGATACCACTGCTTCCCATGT 979 1.6319386564427405 No Hit GGTATCAACGCAGAGTACTTTTTTT 871 1.451908651441907 No Hit TATCAACGCAGAGTACTTTTTTTTT 807 1.3452242040340057 No Hit ACTCTGCGTTGATACCACTGCTTCC 708 1.1801966994499082 No Hit GTGGTATCAACGCAGAGTACATGGG 571 0.9518253042173695 No Hit GTACTTTTTTTTTTTTTTTTTTTTT 515 0.8584764127354559 No Hit GGGAAGCAGTGGTATCAACGCAGAG 489 0.815135855975996 No Hit CATGGGAAGCAGTGGTATCAACGCA 484 0.8068011335222537 No Hit CAGTGGTATCAACGCAGAGTACATG 465 0.775129188198033 No Hit GCAGTGGTATCAACGCAGAGTACAT 453 0.7551258543090515 No Hit GTACTCTGCGTTGATACCACTGCTT 440 0.7334555759293215 No Hit GCAGAGTACATGGGAAGCAGTGGTA 420 0.7001166861143524 No Hit ACATGGGAAGCAGTGGTATCAACGC 412 0.6867811301883647 No Hit GCTTCCCATGTACTCTGCGTTGATA 405 0.6751125187531255 No Hit ACGCAGAGTACTTTTTTTTTTTTTT 376 0.6267711285214203 No Hit AAAAAAAAAAAAAAAAAAAAAAAAA 359 0.5984330721786965 No Hit ATACCACTGCTTCCCATGTACTCTG 344 0.5734289048174696 No Hit CTTATACACATCTCCGAGCCCACGA 336 0.5600933488914819 No Hit AAGCAGTGGTATCAACGCAGAGTAC 325 0.5417569594932489 No Hit GATACCACTGCTTCCCATGTACTCT 324 0.5400900150025004 No Hit CTGCTTCCCATGTACTCTGCGTTGA 323 0.538423070511752 No Hit GTTGATACCACTGCTTCCCATGTAC 316 0.5267544590765127 No Hit ATGGGAAGCAGTGGTATCAACGCAG 313 0.5217536256042674 No Hit TACCACTGCTTCCCATGTACTCTGC 287 0.4784130688448075 No Hit GAGTACTTTTTTTTTTTTTTTTTTT 270 0.4500750125020837 No Hit CCATGTACTCTGCGTTGATACCACT 257 0.42840473412235375 No Hit CCACTGCTTCCCATGTACTCTGCGT 254 0.42340390065010836 No Hit GTACATGGTAAGCAGTGGTATCAAC 244 0.4067344557426238 No Hit CTTCCCATGTACTCTGCGTTGATAC 234 0.39006501083513917 No Hit ATCAACGCAGAGTACATGGGAAGCA 229 0.3817302883813969 No Hit ATACACATCTCCGAGCCCACGAGAC 214 0.35672612102017004 No Hit TTCCCATGTACTCTGCGTTGATACC 205 0.3417236206034339 No Hit GGAAGCAGTGGTATCAACGCAGAGT 176 0.2933822303717286 No Hit TGATACCACTGCTTCCCATGTACTC 176 0.2933822303717286 No Hit GCAGAGTACTTTTTTTTTTTTTTTT 170 0.28338056342723783 No Hit GTACATGGGGAGGAAAAAAAAAAAA 163 0.27171195199199866 No Hit TTTTTTTTTTTTTTTTTTTTTTTTT 161 0.26837806301050177 No Hit GGTATCAACGCAGAGTACATGGGGA 130 0.21670278379729954 No Hit AAAAAGTACTCTGCGTTGATACCAC 130 0.21670278379729954 No Hit ACACATCTCCGAGCCCACGAGACCT 128 0.21336889481580265 No Hit GTGGTATCAACGCAGAGTACTTTTT 125 0.20836806134355723 No Hit ACCATGTACTCTGCGTTGATACCAC 116 0.19336556092682114 No Hit GGTAAGCAGTGGTATCAACGCAGAG 115 0.19169861643607267 No Hit GTACATGGGTAAGCAGTGGTATCAA 112 0.1866977829638273 No Hit TGGGAAGCAGTGGTATCAACGCAGA 109 0.18169694949158194 No Hit AGTGGTATCAACGCAGAGTACATGG 105 0.1750291715285881 No Hit AACGCAGAGTACATGGGAAGCAGTG 94 0.15669278213035506 No Hit TCAACGCAGAGTACATGGGAAGCAG 93 0.15502583763960662 No Hit AAAGTACTCTGCGTTGATACCACTG 88 0.1466911151858643 No Hit ATCAACGCAGAGTACTTTTTTTTTT 88 0.1466911151858643 No Hit GAGTACATGGTAAGCAGTGGTATCA 87 0.14502417069511586 No Hit CCCCATGTACTCTGCGTTGATACCA 86 0.1433572262043674 No Hit CTCTGCGTTGATACCACTGCTTCCC 84 0.14002333722287047 No Hit GGTATCAACGCAGAGTACATGGGGG 82 0.13668944824137358 No Hit CTGCGTTGATACCACTGCTTCCCAT 76 0.12668778129688282 No Hit GTACATGGAAAGCAGTGGTATCAAC 75 0.12502083680613435 No Hit ACTGCTTCCCATGTACTCTGCGTTG 69 0.11501916986164361 No Hit ACGCAGAGTACATGGTAAGCAGTGG 66 0.11001833638939823 No Hit TCCCATGTACTCTGCGTTGATACCA 64 0.10668444740790133 No Hit GTACATGGGGAAAAAAAAAAAAAAA 62 0.10335055842640439 No Hit TTGATACCACTGCTTCCCATGTACT 60 0.10001666944490749 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 0.0 13 0.0 0.0 0.0 0.0 0.0 >>END_MODULE >>Kmer Content warn #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position CTTATAC 35 9.633534E-5 19.026733 1 AAAAAGT 25 0.0059177876 19.026733 1 AAAAGTA 25 0.005946461 19.010853 2 AGTACTC 35 0.0021294823 16.295015 5 AAGTACT 40 0.0051814485 14.258139 4 AAAGTAC 40 0.0051814485 14.258139 3 TTATACA 50 0.001464095 13.307596 2 TATACAC 55 0.002996813 12.097815 3 CAGTGGT 1160 0.0 8.596873 13 GAAGCAG 1105 0.0 8.595021 9 GGAAGCA 1115 0.0 8.517936 8 AGTGGTA 1185 0.0 8.415504 14 GTGGTAT 1210 0.0 8.24163 15 TGGTATC 1215 0.0 8.207714 16 AGCAGTG 1255 0.0 8.021791 11 AAGCAGT 1245 0.0 8.009938 10 GCAGTGG 1285 0.0 7.8345118 12 GGGAAGC 1250 0.0 7.597998 7 TGCGTTG 840 0.0 7.575385 12 CTGCGTT 845 0.0 7.530561 11 >>END_MODULE