FastQCFastQC Report
Thu 26 May 2016
SRR1512620_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512620_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050334
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47800.233132748127866No Hit
GTATCAACGCAGAGTACTTTTTTTT43690.21308723359218548No Hit
GTCCTAAAGTGTGTATTTCTCATTT43100.21020965364667415No Hit
CTGTAGGACGTGGAATATGGCAAGA41940.2045520388385502No Hit
CTTTAGGACGTGAAATATGGCGAGG38130.18596970054635No Hit
GGTATCAACGCAGAGTACTTTTTTT28860.1407575546228078No Hit
TATCAACGCAGAGTACTTTTTTTTT27140.13236867749352058No Hit
GTCCTACAGTGTGCATTTCTCATTT26220.1278816036801809No Hit
GTACATGGGAAGCAGTGGTATCAAC24570.11983413434103907No Hit
CTGAAGGACCTGGAATATGGCGAGA20620.10056898046854805No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATGGCG759.92597E-713.90196916
TAGGACC8300.013.0140324
TACGACT658.2037156E-411.6616974
GTCTTAT1851.8189894E-1211.3914361
GGCGAGG10400.011.28537419
TCCAACG2350.011.27569418
CCAACGA1701.2732926E-1111.13547219
TGGCGAA855.5372257E-511.13356418
GGACCTG15250.010.9352316
AGGACCT15600.010.8721045
CGTGCGC700.001529719810.82447310
CCACCTT6450.010.72405213
GACGTGG12500.010.6879457
AAGGCGT1355.9122613E-810.5279216
GGACGTG24850.010.4855566
TCGAACT1451.5288606E-810.44430619
AGGACGT25300.010.4114075
GAACAGT5050.010.3194486
TGTAGAA9100.010.3081072
GTAGAAC8150.010.2308143
Produced by FastQC (version 0.11.5)