FastQCFastQC Report
Thu 26 May 2016
SRR1512620_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512620_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2050334
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT56770.2768817178079279No Hit
GTCCTACAGTGGACATTTCTAAATT51790.2525929921661544No Hit
CTGTAGGACGTGGAATATGGCAAGA50130.24449675028556322No Hit
GTCCTAAAGTGTGTATTTCTCATTT46300.22581686691046432No Hit
CTTTAGGACGTGAAATATGGCGAGG45220.22054943243393516No Hit
TATCAACGCAGAGTACTTTTTTTTT39020.19031045673534164No Hit
GGTATCAACGCAGAGTACTTTTTTT38770.18909114319910802No Hit
GTCCTACAGTGTGCATTTCTCATTT29730.14500076572890075No Hit
GTACATGGGAAGCAGTGGTATCAAC29520.14397654235846452No Hit
CTGAAGGACCTGGAATATGGCGAGA24490.11944395400944431No Hit
CTGTAGGACCTGGAATATGGCGAGA23000.11217684533349201No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AACCGGG250.00603321218.9997337
AATACCG500.001497511513.3027325
TAGGACC9350.013.1093254
GCACCGT550.003068222712.0913296
GGCGAGG13050.011.425055519
CTAGGAC2800.011.1984563
GTCGAGG951.3660438E-510.99608819
TTAGGAC17650.010.874493
TATGTCG1408.390998E-910.85407616
TGTCGAG1502.1445885E-910.76336418
GCGCCAC803.776527E-410.68604613
CCAACGA1451.4519173E-810.47903219
AGGACGT28300.010.4756515
GGACGTG27800.010.3546766
GACGTGA15000.010.3231877
TGGCGAG29350.010.28985718
GGACCTG18300.010.2790456
GATATAC5650.010.2710251
AGGACCT18800.010.2095445
AAATGTC7300.010.1505427
Produced by FastQC (version 0.11.5)