FastQCFastQC Report
Thu 26 May 2016
SRR1512619_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512619_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418992
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT62670.2590748543194851No Hit
CTGTAGGACGTGGAATATGGCAAGA56810.23484988788718608No Hit
GTCCTAAAGTGTGTATTTCTCATTT56730.23451917162189873No Hit
CTTTAGGACGTGAAATATGGCGAGG50010.20673900533776055No Hit
GTATCAACGCAGAGTACTTTTTTTT39300.1624643653224153No Hit
GTCCTACAGTGTGCATTTCTCATTT35970.14869830077982896No Hit
CTGTAGGACCTGGAATATGGCGAGA26850.11099664653706999No Hit
CTGAAGGACCTGGAATATGGCGAGA26000.1074827862183918No Hit
GGTATCAACGCAGAGTACTTTTTTT25710.10628393975672512No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGTAGG653.2440403E-513.9016381
TAGGACC11300.013.4989194
TAGGGGT1354.9367372E-911.2289134
GATATAC6150.011.1012181
GTACCGT600.00599555411.0536926
GGCGAGG13900.011.03201319
AGGACCT20450.011.0264365
ACCTTTT9450.010.73364515
AAGTCGT2400.010.6589177
TCCAACG2500.010.60030718
CCACCTT9700.010.54990913
TGGCGAA909.886659E-510.51617918
CACCTTT9650.010.51118714
ATAGGAC4150.010.50173953
AGGACGT34850.010.4938795
GGACCTG20100.010.4173386
GTAGGAC36150.010.4047993
GGACGTG34350.010.3986076
ACCGTCC1002.5407062E-510.3961248
TTTAGAA11050.010.3744622
Produced by FastQC (version 0.11.5)