FastQCFastQC Report
Thu 26 May 2016
SRR1512619_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512619_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2418992
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT67870.2805714115631635No Hit
CTGTAGGACGTGGAATATGGCAAGA65540.27093930033666913No Hit
GTCCTAAAGTGTGTATTTCTCATTT61870.25576769166661156No Hit
CTTTAGGACGTGAAATATGGCGAGG59870.24749978503442754No Hit
GTATCAACGCAGAGTACTTTTTTTT52340.21637111656425487No Hit
GTCCTACAGTGTGCATTTCTCATTT39420.16296043972034632No Hit
TATCAACGCAGAGTACTTTTTTTTT33690.13927288721913922No Hit
GGTATCAACGCAGAGTACTTTTTTT33590.13885949188753002No Hit
CTGTAGGACCTGGAATATGGCGAGA31620.13071560385482878No Hit
CTGAAGGACCTGGAATATGGCGAGA30880.1276564784009207No Hit
ATTTAGAAATGTCCACTGTAGGACG25300.1045890188971274No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGACCGT550.003068694412.0912246
AATGCCG2750.012.08672518
TAGGACC12950.011.4466814
ACCGTCC752.0750189E-411.3998268
GAGGCGA855.324048E-511.1767636
ATTTAGA9450.011.17427351
GGCGAGG15750.011.15440819
GTACGAC600.005867980411.0856873
AATGTCC9500.010.7998358
ATGCCGG3100.010.72165219
GTCCTAA17850.010.7123791
ACTGTTC8100.010.6726778
AGGACGT37250.010.6373775
TAGAAAT9900.010.4620334
AGGACCT24350.010.4582885
TTTAGAA11200.010.4354672
TGTAGGA39000.010.4280692
TTAGGAC21550.010.3618273
GATATAC7900.010.3561771
GGACGTG37350.010.3523456
Produced by FastQC (version 0.11.5)