FastQCFastQC Report
Thu 26 May 2016
SRR1512618_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512618_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051457
Sequences flagged as poor quality0
Sequence length25
%GC45

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTCCTACAGTGGACATTTCTAAATT47000.2291054601680659No Hit
GTCCTAAAGTGTGTATTTCTCATTT40180.1958607955224019No Hit
CTGTAGGACGTGGAATATGGCAAGA39440.19225360317081955No Hit
CTTTAGGACGTGAAATATGGCGAGG32730.159545142793634No Hit
GTATCAACGCAGAGTACTTTTTTTT30810.15018594101655555No Hit
GTCCTACAGTGTGCATTTCTCATTT25190.12279077748156556No Hit
TTGTAGAACAGTGTATATCAATGAG20830.10153759011278325No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG350.002210905316.2408185
TCCAACG4600.013.38428818
TCACGAC500.001531069313.26365953
TAGGACC6700.013.0088154
CGGTTTC3700.012.03812913
CCAACGA2600.012.02298219
CGTGCGC855.495474E-511.14184410
ACCGTGC855.5712117E-511.1268788
GCCGGTT4000.010.89168511
CCAACGT3050.010.87025719
GGCGAGG10100.010.78569419
GTCCTAC24000.010.7083751
CCGGTTT4100.010.62811312
TGCCGGT4250.010.25049710
GATATAC10200.010.1961171
AGGACCT14850.010.1436775
TGTAGGA26550.010.1341922
GGACGTG24400.010.1338726
AGGACGT25250.010.0928925
GGACCTG15050.010.0718266
Produced by FastQC (version 0.11.5)