FastQCFastQC Report
Thu 26 May 2016
SRR1512618_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512618_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences2051457
Sequences flagged as poor quality0
Sequence length25
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CTGTAGGACGTGGAATATGGCAAGA47630.2321764482511698No Hit
GTATCAACGCAGAGTACTTTTTTTT43190.2105332941416759No Hit
GTCCTACAGTGGACATTTCTAAATT42370.20653613504938195No Hit
CTTTAGGACGTGAAATATGGCGAGG37500.18279690970856324No Hit
GTCCTAAAGTGTGTATTTCTCATTT36570.17826354634779087No Hit
TATCAACGCAGAGTACTTTTTTTTT28570.1392668722766307No Hit
GGTATCAACGCAGAGTACTTTTTTT28210.13751202194342851No Hit
TTGTAGAACAGTGTATATCAATGAG26820.1307363498235644No Hit
GTCCTACAGTGTGCATTTCTCATTT22630.11031184177879429No Hit
CTGAAGGACCTGGAATATGGCGAGA22400.10919068739924845No Hit
GATATACACTGTTCTACAAATCCCG21630.10543725751989927No Hit
GTGTATATCAATGAGTTACAATGAA21110.10290247370527386No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCCAACG4300.013.69389618
CCAACGA2900.013.099833519
GGCGAGG10250.012.23077119
TAGGACC9850.011.575494
AACCGTG752.0745803E-411.3999127
CGTTTCC2800.011.19361314
TGGACCG855.3117605E-511.1791115
GATATAC10050.010.9779371
CCAACGT2200.010.79247719
GCCGGTT2650.010.75437211
TGGCGAG24600.010.73281118
GGACCGT803.772901E-410.6871586
GTGCGCC803.772901E-410.68715811
CCGTTTC3050.010.58930813
ACTGTTC10600.010.3958938
CCCGTTT3300.010.36305112
TCGAACT1201.5295227E-610.28882819
GACGTGA12300.010.2722787
GGACCTG17100.010.2218946
TTTAGAA8100.010.2053672
Produced by FastQC (version 0.11.5)