FastQCFastQC Report
Thu 26 May 2016
SRR1512617_2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512617_2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829215
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[FAIL]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[WARN]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT35680.19505634930830984No Hit
GTACATGGGAAGCAGTGGTATCAAC25690.1404427582323565No Hit
GGTATCAACGCAGAGTACTTTTTTT22850.12491697258113453No Hit
TATCAACGCAGAGTACTTTTTTTTT21690.11857545449824106No Hit
GTCCTACAGTGGACATTTCTAAATT20330.1111405712286418No Hit
CCCATGTACTCTGCGTTGATACCAC18890.10326834188436024No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGGACC4100.012.4795284
TCGGTGG701.14471E-412.1516718
TGCGAAA550.003135904712.05602812
GGCGAGT658.2784134E-411.64897419
CTACACT3000.011.3702374
TCCAACG1501.891749E-1011.35619318
TTAGGAC7700.010.7060363
TGTGCGC1252.3400389E-710.60639110
GTCTTAG2650.010.6024481
GTCTATA1801.4551915E-1010.5919291
TGGCGAG11650.010.56012218
AGGACGT10850.010.4794815
GTACTAG1251.2744222E-610.4358381
GTCTAAA1654.1618478E-910.3385341
GGACGTG11100.010.3288186
TATACAG4050.010.2940425
GTAGGAC11700.010.2853263
TGTAGGA12550.010.2682262
TCGAACT1201.5980859E-610.25352619
GGCGAGG5650.010.21862619
Produced by FastQC (version 0.11.5)