##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR1512617_2.fastq.gz File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 1829215 Sequences flagged as poor quality 0 Sequence length 25 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 26.27173514321717 31.0 25.0 33.0 7.0 34.0 2 27.890538291015545 31.0 27.0 34.0 16.0 34.0 3 29.032431398168068 31.0 28.0 34.0 19.0 34.0 4 32.82500307508959 35.0 33.0 37.0 26.0 37.0 5 33.12131870775168 37.0 35.0 37.0 27.0 37.0 6 33.330276102043776 37.0 35.0 37.0 28.0 37.0 7 33.28726311559877 37.0 35.0 37.0 27.0 37.0 8 33.31279155266057 37.0 35.0 37.0 27.0 37.0 9 34.7819884485968 39.0 35.0 39.0 27.0 39.0 10 34.69794201337732 39.0 35.0 39.0 27.0 39.0 11 34.7462211932441 39.0 35.0 39.0 27.0 39.0 12 34.66841677987552 39.0 35.0 39.0 27.0 39.0 13 34.63866467309747 39.0 35.0 39.0 27.0 39.0 14 35.73821119988629 40.0 36.0 41.0 27.0 41.0 15 35.65267450791733 40.0 36.0 41.0 27.0 41.0 16 35.49729146109123 40.0 36.0 41.0 26.0 41.0 17 35.43429667917658 40.0 36.0 41.0 25.0 41.0 18 35.384987002621344 40.0 36.0 41.0 25.0 41.0 19 35.38777180375188 40.0 36.0 41.0 25.0 41.0 20 35.17676216300435 40.0 36.0 41.0 24.0 41.0 21 35.15957828904749 40.0 36.0 41.0 24.0 41.0 22 35.06166688989539 40.0 36.0 41.0 23.0 41.0 23 34.91089511074423 40.0 36.0 41.0 19.0 41.0 24 34.77667032032866 40.0 35.0 41.0 18.0 41.0 25 34.64246193039091 40.0 35.0 41.0 18.0 41.0 >>END_MODULE >>Per tile sequence quality fail #Tile Base Mean 1101 1 -1.0459588367288006 1101 2 -2.7241025489219375 1101 3 -3.4958695788912024 1101 4 -3.9581955584463344 1101 5 -4.032421436847898 1101 6 -4.222040110309166 1101 7 -4.078302423849191 1101 8 -4.238729201065091 1101 9 -4.473588241940249 1101 10 -5.135870284342943 1101 11 -4.963472843143354 1101 12 -4.726998135844621 1101 13 -4.868736437456839 1101 14 -6.147207392258469 1101 15 -5.9768935400491365 1101 16 -5.988206946453971 1101 17 -6.0925198701997125 1101 18 -6.232679623840973 1101 19 -6.017797225423717 1101 20 -6.246962402979456 1101 21 -6.3512726052810855 1101 22 -6.790223231235341 1101 23 -6.8715109340920115 1101 24 -7.29761329582611 1101 25 -7.51934108081436 1102 1 0.5004728398687099 1102 2 -0.1595225557817983 1102 3 -0.6845579897170211 1102 4 -1.354326119120195 1102 5 -1.269129661500319 1102 6 -1.0410506952318315 1102 7 -1.1480679921406889 1102 8 -1.154211744318097 1102 9 -1.4302283465929904 1102 10 -1.4219064820664258 1102 11 -1.4164652574150551 1102 12 -1.5882670503117424 1102 13 -1.6047897848289523 1102 14 -1.7119525006203204 1102 15 -1.9690653236923623 1102 16 -1.9566811063206018 1102 17 -1.9036264612483933 1102 18 -1.9991931401570682 1102 19 -2.0136845003844286 1102 20 -2.1569395101926787 1102 21 -2.107085538509132 1102 22 -2.1773474345743367 1102 23 -2.4267862457769027 1102 24 -2.6251939191161284 1102 25 -2.7055264405980353 1103 1 2.4074468880753344 1103 2 0.7921136045851043 1103 3 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0.6171187185364957 >>END_MODULE >>Per sequence quality scores pass #Quality Count 2 85064.0 3 17.0 4 778.0 5 3140.0 6 3761.0 7 3457.0 8 2863.0 9 2812.0 10 2817.0 11 2772.0 12 2849.0 13 3028.0 14 3119.0 15 2949.0 16 3143.0 17 3410.0 18 3669.0 19 4568.0 20 5948.0 21 6926.0 22 8485.0 23 9878.0 24 11475.0 25 13038.0 26 15237.0 27 17402.0 28 19787.0 29 23904.0 30 29535.0 31 37704.0 32 49455.0 33 66695.0 34 92833.0 35 135636.0 36 210853.0 37 273998.0 38 666210.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 43.78840288150831 19.419052243018662 12.052610060190165 24.739934815282865 2 15.447027540485731 19.95663554023155 40.24606427537637 24.35027264390635 3 19.075685898836802 24.129902182289623 29.495936722747945 27.298475196125633 4 12.885417317038709 16.62378318814291 37.49045664606288 33.0003428487555 5 13.913306299272096 37.32567575114793 35.329440662133734 13.431577287446245 6 32.7339640926275 36.23436591818098 17.789504765755147 13.242165223436372 7 28.508920220777114 31.297559508388918 21.93785923483255 18.25566103600142 8 25.84172084419351 35.91122963339843 20.4101336555658 17.836915866842258 9 26.838442813317563 15.015963585707413 19.95568776037414 38.189905840600886 10 15.573964043183244 28.46571282211429 34.15075784346759 21.809565291234875 11 34.66367690894259 22.27378635138711 24.15787060294969 18.904666136720603 12 23.68513329746445 24.463144722178694 31.1042392856321 20.747482694724752 13 29.21884101570998 21.577836703671615 25.466836101443867 23.736486179174538 14 21.726466283012364 21.616203576084832 27.307556776799675 29.349773364103132 15 23.909802807446304 29.79601753748984 23.149804694141483 23.144374960922377 16 22.964278082621455 27.59907999404534 27.04981204917758 22.386829874155623 17 22.649527103641653 27.92417836455211 27.309654820817986 22.11663971098825 18 22.55133770579457 27.146147288979073 28.706603176133637 21.595911829092717 19 24.066264812770957 26.64362533925251 27.233863083028197 22.056246764948334 20 24.10753652860369 26.931893057196426 27.478185808547778 21.482384605652108 21 23.724538145262805 26.319174133724516 27.024212658593953 22.932075062418726 22 23.763295145935505 27.268192189070927 26.772951086581127 22.19556157841244 23 23.141400255229186 27.062071080780587 27.10418937348355 22.69233929050668 24 23.589474480748287 26.64368020301785 27.534844342379866 22.232000973853992 25 23.45913547122178 26.614072497008866 27.336497175637138 22.590294856132218 >>END_MODULE >>Per sequence GC content pass #GC Content Count 0 1418.0 1 1418.0 2 1335.0 3 1252.0 4 1252.0 5 1252.0 6 1938.0 7 2624.0 8 2624.0 9 2624.0 10 3429.5 11 4235.0 12 4235.0 13 4235.0 14 6666.5 15 9098.0 16 9098.0 17 9098.0 18 15416.5 19 21735.0 20 21735.0 21 21735.0 22 33191.0 23 44647.0 24 44647.0 25 44647.0 26 62962.0 27 81277.0 28 81277.0 29 81277.0 30 103931.0 31 126585.0 32 126585.0 33 126585.0 34 143772.5 35 160960.0 36 160960.0 37 160960.0 38 175642.5 39 190325.0 40 190325.0 41 190325.0 42 203514.0 43 216703.0 44 216703.0 45 216703.0 46 227082.0 47 237461.0 48 237461.0 49 237461.0 50 231021.0 51 224581.0 52 224581.0 53 224581.0 54 203970.0 55 183359.0 56 183359.0 57 183359.0 58 159845.5 59 136332.0 60 136332.0 61 136332.0 62 112587.0 63 88842.0 64 88842.0 65 88842.0 66 69430.5 67 50019.0 68 50019.0 69 50019.0 70 37544.5 71 25070.0 72 25070.0 73 25070.0 74 17936.5 75 10803.0 76 10803.0 77 10803.0 78 7761.0 79 4719.0 80 4719.0 81 4719.0 82 3518.5 83 2318.0 84 2318.0 85 2318.0 86 1907.0 87 1496.0 88 1496.0 89 1496.0 90 1361.0 91 1226.0 92 1226.0 93 1226.0 94 1171.0 95 1116.0 96 1116.0 97 1116.0 98 1065.0 99 1014.0 100 1014.0 >>END_MODULE >>Per base N content warn #Base N-Count 1 5.868036288790546 2 0.2053886503226794 3 0.18980819641212215 4 0.18242798140185817 5 0.1572805821076254 6 0.20752071243675566 7 0.39836760577624825 8 0.28826573147497697 9 0.24256306667067568 10 0.2170329895610959 11 0.2116755001462376 12 0.22135178204858366 13 0.23638555336578806 14 0.24502313834076367 15 0.32369076352424403 16 0.48064333607585763 17 0.4888982432354863 18 0.21052746670019654 19 0.1918855902668631 20 0.18882416774408695 21 0.1990471322397859 22 0.27623871442121345 23 0.1861454230366578 24 0.30247947890215204 25 0.3458314085550359 >>END_MODULE >>Sequence Length Distribution pass #Length Count 25 1829215.0 >>END_MODULE >>Sequence Duplication Levels pass #Total Deduplicated Percentage 73.17772385654925 #Duplication Level Percentage of deduplicated Percentage of total 1 84.08815582204807 61.53379846352317 2 10.606876069745137 15.523740960249002 3 2.798589732091167 6.143832798082247 4 1.0315141920416349 3.0193544279733713 5 0.4782552188052235 1.7498814167341095 6 0.276266229052677 1.2129920292304188 7 0.16589306506932752 0.8497773833751863 8 0.11083617393090556 0.6488591143385816 9 0.08045610793530687 0.5298835364156347 >10 0.3332962080191811 4.106894023325739 >50 0.01582366079828614 0.8170416531082574 >100 0.01141091509217774 1.7513475886271266 >500 0.001651009090264703 0.7927797600672422 >1k 9.755962806109596E-4 1.3198168449498484 >5k 0.0 0.0 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences warn #Sequence Count Percentage Possible Source GTATCAACGCAGAGTACTTTTTTTT 3568 0.19505634930830984 No Hit GTACATGGGAAGCAGTGGTATCAAC 2569 0.1404427582323565 No Hit GGTATCAACGCAGAGTACTTTTTTT 2285 0.12491697258113453 No Hit TATCAACGCAGAGTACTTTTTTTTT 2169 0.11857545449824106 No Hit GTCCTACAGTGGACATTTCTAAATT 2033 0.1111405712286418 No Hit CCCATGTACTCTGCGTTGATACCAC 1889 0.10326834188436024 No Hit >>END_MODULE >>Adapter Content pass #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 0.0 0.0 0.0 0.0 0.0 2 0.0 0.0 0.0 0.0 0.0 3 0.0 0.0 0.0 0.0 0.0 4 0.0 0.0 0.0 0.0 0.0 5 0.0 0.0 0.0 0.0 0.0 6 0.0 0.0 0.0 0.0 0.0 7 0.0 0.0 0.0 0.0 0.0 8 0.0 0.0 0.0 0.0 0.0 9 0.0 0.0 0.0 0.0 0.0 10 0.0 0.0 0.0 0.0 0.0 11 0.0 0.0 0.0 0.0 0.0 12 0.0 0.0 0.0 0.0 5.4668259335288635E-5 13 0.0 0.0 0.0 5.4668259335288635E-5 1.0933651867057727E-4 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TAGGACC 410 0.0 12.479528 4 TCGGTGG 70 1.14471E-4 12.151671 8 TGCGAAA 55 0.0031359047 12.056028 12 GGCGAGT 65 8.2784134E-4 11.648974 19 CTACACT 300 0.0 11.370237 4 TCCAACG 150 1.891749E-10 11.356193 18 TTAGGAC 770 0.0 10.706036 3 TGTGCGC 125 2.3400389E-7 10.606391 10 GTCTTAG 265 0.0 10.602448 1 GTCTATA 180 1.4551915E-10 10.591929 1 TGGCGAG 1165 0.0 10.560122 18 AGGACGT 1085 0.0 10.479481 5 GTACTAG 125 1.2744222E-6 10.435838 1 GTCTAAA 165 4.1618478E-9 10.338534 1 GGACGTG 1110 0.0 10.328818 6 TATACAG 405 0.0 10.294042 5 GTAGGAC 1170 0.0 10.285326 3 TGTAGGA 1255 0.0 10.268226 2 TCGAACT 120 1.5980859E-6 10.253526 19 GGCGAGG 565 0.0 10.218626 19 >>END_MODULE