FastQCFastQC Report
Thu 26 May 2016
SRR1512617_1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR1512617_1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1829215
Sequences flagged as poor quality0
Sequence length25
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT47680.2606582605106562No Hit
GTACATGGGAAGCAGTGGTATCAAC32040.17515710291026476No Hit
GGTATCAACGCAGAGTACTTTTTTT31780.1737357281675473No Hit
TATCAACGCAGAGTACTTTTTTTTT30150.16482480189589524No Hit
CCCATGTACTCTGCGTTGATACCAC22020.12037950705630557No Hit
GTCCTACAGTGGACATTTCTAAATT21560.11786476712688229No Hit
CTGTAGGACGTGGAATATGGCAAGA20650.11288995552737104No Hit
GTCCTAAAGTGTGTATTTCTCATTT19690.10764180263118331No Hit
CTTTAGGACGTGAAATATGGCGAGG18740.1024483179943309No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGCCAA508.7195614E-515.19912511
TAGGACC5300.013.447774
TCCAACG951.0449512E-611.99537318
CTGTGCG752.0747789E-411.3996559
GTGCGTT752.075306E-411.399343511
AGGACCT9500.010.9044425
GTAGGAC13900.010.801783
GTATAGG1803.45608E-1110.5688211
TGTAGGA14250.010.5364732
GTCTAGA1951.4551915E-1110.2436281
TAACACT2053.6379788E-1210.198444
CCAACGA750.002658247210.12859519
TAATACT2350.010.1096734
GGACGTG11850.010.1028946
AGGACGT12050.010.0165285
ACCGTGC1054.1066483E-59.9520818
AATGTCC4200.09.9520818
GGCGAGG5000.09.8753819
GGACCTG9200.09.8113646
GTCCTAC11600.09.7579381
Produced by FastQC (version 0.11.5)